High throughput screening of bradykinin-potentiating peptides in Bothrops moojeni snake venom using precursor ion mass spectrometry
| Autor: | Frédéric Perret, Laure Menin, Sophie Michalet, Anna Maria Perchuc, Reto Stöcklin, Reto Schöni, Marianne Wilmer, Philippe Favreau |
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| Rok vydání: | 2007 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Electrospray ionization Peptide Venom Toxicology Mass spectrometry Tandem mass spectrometry Bradykinin Peptide Mapping Mass Spectrometry Bothrops moojeni Tandem Mass Spectrometry Crotalid Venoms Animals Bothrops chemistry.chemical_classification Chromatography biology Chemistry biology.organism_classification Peptide Fragments Pyrrolidonecarboxylic Acid Matrix-assisted laser desorption/ionization Snake venom Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Peptides |
| Zdroj: | Toxicon : official journal of the International Society on Toxinology. 51(7) |
| ISSN: | 0041-0101 |
| Popis: | Snake venoms are known to be an extensive source of bioactive peptides. Bradykinin-potentiating peptides (BPPs) are inhibitors of the angiotensin-converting enzyme that have already been identified in the venom of many snake, scorpion, spider and batrachian species. Their most characteristic structural features are an invariable N-terminal pyroglutamate residue (pGlu or Z) and two consecutive proline residues at the C-terminus. Fragmentation of BPPs by collision-induced dissociation during electrospray tandem mass spectrometry analysis (ESI-MS/MS) generates a predominant signal at m / z 213.1 corresponding to the y -ion of the terminal Pro–Pro fragment. In addition, signals at m / z 226.1 and 240.1 that correspond to the b ions of the N-terminus pGlu–Asn and pGlu–Lys, respectively, can often be observed. Based on these structural determinants, the present work describes an original methodology for the discovery of BPPs in natural extracts using liquid chromatography coupled to ESI-MS/MS operated in precursor ion-scan mode. The venom of the Bothrops moojeni snake was used as a model and the methodology was applied for subsequent structural analysis of the identified precursors by tandem mass spectrometry on quadrupole-time-of-flight (Q-TOF) and matrix-assisted laser-desorption/ionization time-of-flight tandem mass spectrometry (MALDI-TOF-MS/MS) instruments. More than 40 peptides below 2500 Da could be detected, among them 20 were shown to belong to the BPP-like family including the related tripeptides pGlu–Lys–Trp and pGlu–Asn–Trp. A total of 15 new sequences have been identified using this approach. |
| Databáze: | OpenAIRE |
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