The effect of C. burnetii infection on the cytokine response of PBMCs from pregnant goats

Autor: Johanna M.J. Rebel, Teske Schoffelen, Jacob Post, Marcel van Deuren, Tom Sprong, Hendrik I.J. Roest, Annemieke Dinkla, Anne Ammerdorffer
Rok vydání: 2014
Předmět:
Epidemiology
interleukin-10
Interleukin-1beta
lnfectious Diseases and Global Health Radboud Institute for Molecular Life Sciences [Radboudumc 4]
lcsh:Medicine
Pathogenesis
Pathology and Laboratory Medicine
factor-alpha
Pregnancy
Zoonoses
Medicine and Health Sciences
Medicine
Interferon gamma
Pregnancy Complications
Infectious
lcsh:Science
Immune Response
t-cells
Innate Immune System
Multidisciplinary
biology
Goats
Bacteriologie
Toll-Like Receptors
Interleukin
Bacteriology
Host Pathogen Interaction & Diagnostics
interferon
Veterinary Bacteriology
Bacterial Pathogens
Interleukin-10
Interleukin 10
Infectious Diseases
Veterinary Diseases
Medical Microbiology
Coxiella burnetii
Host-Pathogen Interactions
Cytokines
Female
monocytes
Q Fever
Research Article
medicine.drug
Veterinary Medicine
mice
q-fever endocarditis
Bioinformatica & Diermodellen
Immunology
Q fever
Microbiology
Peripheral blood mononuclear cell
Veterinary Immunology
Interferon-gamma
Immune system
Bio-informatics & Animal models
Animals
Epidemiology
Bio-informatics & Animal models
Gram Negative Bacteria
Microbial Pathogens
Host Pathogen Interaction & Diagnostics
Epidemiologie
Goat Diseases
Tumor Necrosis Factor-alpha
business.industry
lcsh:R
Immunity
Biology and Life Sciences
Bacteriology
Molecular Development
biology.organism_classification
medicine.disease
bacterial infections and mycoses
Host Pathogen Interactie & Diagnostiek
Chronic infection
Emerging Infectious Diseases
lnfectious Diseases and Global Health Radboud Institute for Health Sciences [Radboudumc 4]
Gene Expression Regulation
Epidemiologie
Bioinformatica & Diermodellen
Immune System
Bacteriologie
Host Pathogen Interactie & Diagnostiek
WIAS
Leukocytes
Mononuclear
bacteria
lcsh:Q
gamma
Veterinary Science
tumor-necrosis-factor
business
Developmental Biology
Zdroj: PLoS One, 9, 10
PLoS ONE
PLoS One, 9
PLoS ONE, 9(10)
PLoS ONE, Vol 9, Iss 10, p e109283 (2014)
PLoS ONE 9 (2014) 10
ISSN: 1932-6203
Popis: Contains fulltext : 138727.pdf (Publisher’s version ) (Open Access) In humans, infection with Coxiella burnetii, the causative agent of Q fever, leads to acute or chronic infection, both associated with specific clinical symptoms. In contrast, no symptoms are observed in goats during C. burnetii infection, although infection of the placenta eventually leads to premature delivery, stillbirth and abortion. It is unknown whether these differences in clinical outcome are due to the early immune responses of the goats. Therefore, peripheral blood mononuclear cells (PBMCs) were isolated from pregnant goats. In total, 17 goats were included in the study. Six goats remained naive, while eleven goats were infected with C. burnetii. Toll-like receptor (TLR) and cytokine mRNA expression were measured after in vitro stimulation with heat-killed C. burnetii at different time points (prior infection, day 7, 35 and 56 after infection). In naive goats an increased expression of interleukin (IL)-1beta, tumor necrosis factor (TNF)-alpha, IL-10 and interferon (IFN)-gamma mRNA upon C. burnetii stimulation was detected. In addition, TLR2 expression was strongly up-regulated. In goats infected with C. burnetii, PBMCs re-stimulated in vitro with C. burnetii, expressed significantly more TNF-alpha mRNA and IFN-gamma mRNA compared to naive goats. In contrast, IL-10 mRNA production capacity was down-regulated during C. burnetii infection. Interestingly, at day 7 after inoculation a decreased IFN-gamma protein level was observed in stimulated leukocytes in whole blood from infected goats, whereas at other time-points increased production of IFN-gamma protein was seen. Our study shows that goats initiate a robust pro-inflammatory immune response against C. burnetii in vitro. Furthermore, PBMCs from C. burnetii infected goats show augmented pro-inflammatory cytokine responses compared to PBMCs from non-infected goats. However, despite this pro-inflammatory response, goats are not capable of clearing the C. burnetii infection.
Databáze: OpenAIRE
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