Cadmium (II)-Induced Oxidative Stress Results in Replication Stress and Epigenetic Modifications in Root Meristem Cell Nuclei of Vicia faba
Autor: | Mateusz Wróblewski, Janusz Maszewski, Aneta Żabka, Justyna Teresa Polit, Konrad Winnicki |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
0106 biological sciences
0301 basic medicine Epigenomics DNA damage DNA repair cadmium epigenetic modifications replication stress Meristem 01 natural sciences Article 03 medical and health sciences Histone H3 H3S10Ph Nucleosome lcsh:QH301-705.5 chromatin structure Cell Nucleus biology Chemistry DNA replication General Medicine Cell cycle Chromatin Cell biology gamma-H2AX Vicia faba Oxidative Stress 030104 developmental biology Histone H4K5Ac lcsh:Biology (General) biology.protein 010606 plant biology & botany |
Zdroj: | Cells Volume 10 Issue 3 Cells, Vol 10, Iss 640, p 640 (2021) |
ISSN: | 2073-4409 |
Popis: | Among heavy metals, cadmium is considered one of the most toxic and dangerous environmental factors, contributing to stress by disturbing the delicate balance between production and scavenging of reactive oxygen species (ROS). To explore possible relationships and linkages between Cd(II)-induced oxidative stress and the consequent damage at the genomic level (followed by DNA replication stress), root apical meristem (RAM) cells in broad bean (V. faba) seedlings exposed to CdCl2 treatment and to post-cadmium recovery water incubations were tested with respect to H2O2 production, DNA double-strand breaks (γ-phosphorylation of H2AX histones), chromatin morphology, histone H3S10 phosphorylation on serine (a marker of chromatin condensation), mitotic activity, and EdU staining (to quantify cells typical of different stages of nuclear DNA replication). In order to evaluate Cd(II)-mediated epigenetic changes involved in transcription and in the assembly of nucleosomes during the S-phase of the cell cycle, the acetylation of histone H3 on lysine 5 (H3K56Ac) was investigated by immunofluorescence. Cellular responses to cadmium (II) toxicity seem to be composed of a series of interlinked biochemical reactions, which, via generation of ROS and DNA damage-induced replication stress, ultimately activate signal factors engaged in cell cycle control pathways, DNA repair systems, and epigenetic adaptations. |
Databáze: | OpenAIRE |
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