Production of a Rhizopus oryzae lipase from Pichia pastoris using alternative operational strategies
| Autor: | José Luis Montesinos, Mehmedalija Jahic, Francisco Valero, Anna Surribas, Sven-Olof Enfors, Rainer Stahn |
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| Rok vydání: | 2006 |
| Předmět: |
Microbiological Techniques
Time Factors Lipolysis Triacylglycerol lipase Rhizopus oryzae Cell Culture Techniques Bioengineering Applied Microbiology and Biotechnology Pichia Pichia pastoris chemistry.chemical_compound Bioreactors Bioreactor Food science Biomass Lipase biology Methanol Temperature High cell General Medicine biology.organism_classification Alcohol Oxidoreductases chemistry Biochemistry Fermentation biology.protein Salts Rhizopus Biotechnology |
| Zdroj: | Journal of biotechnology. 130(3) |
| ISSN: | 0168-1656 |
| Popis: | Different cultivation strategies have been compared for the production of Rhizopus oryzae lipase (ROL) from Pichia pastoris. Several drawbacks have been found using a methanol non-limited fed-batch. On the one hand, oxygen limitation appeared at early cell dry weights and, on the other hand, high cell death was observed. A temperature limited fed-batch has been proposed to solve both problems. However, in our case study a methanol non-limited fed-batch results in better productivities. Finally, a lower salt medium were used to overcome cell death problems and a temperature limited fed-batch was applied thereafter to solve oxygen transfer limitations. This combined strategy has resulted in lower productivities when compared to a methanol non-limited fed-batch. However the culture could be longer prolonged and a 1.3-fold purer final product was obtained mainly due to cell death reduction. |
| Databáze: | OpenAIRE |
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