Zinc‐oxide nanoparticles act catalytically and synergistically with nitric oxide donors to enhance antimicrobial efficacy
| Autor: | Priyadarshini Singha, Sean P. Hopkins, Christina D. Workman, Jitendra Pant, Hitesh Handa |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Materials science
Cell Survival 0206 medical engineering Kinetics Biomedical Engineering Nanoparticle chemistry.chemical_element Microbial Sensitivity Tests 02 engineering and technology Zinc S-Nitroso-N-Acetylpenicillamine Nitric Oxide Bacterial Adhesion Catalysis Article Nitric oxide Biomaterials Mice chemistry.chemical_compound Animals Nitric Oxide Donors Microbial Viability Metals and Alloys Snap Spectrometry X-Ray Emission 3T3 Cells 021001 nanoscience & nanotechnology Antimicrobial 020601 biomedical engineering Anti-Bacterial Agents chemistry Toxicity Ceramics and Composites Nanoparticles Zinc Oxide 0210 nano-technology Nuclear chemistry |
| Zdroj: | J Biomed Mater Res A |
| ISSN: | 1552-4965 1549-3296 |
| DOI: | 10.1002/jbm.a.36657 |
| Popis: | The development of infection-resistant materials is of substantial importance as seen with an increase in antibiotic resistance. In this project, the nitric oxide (NO)-releasing polymer has an added topcoat of zinc oxide nanoparticle (ZnO-NP) to improve NO-release and match the endogenous NO flux (0.5-4 × 10-10 mol cm-2 min-1 ). The ZnO-NP is incorporated to act as a catalyst and provide the additional benefit of acting synergistically with NO as an antimicrobial agent. The ZnO-NP topcoat is applied on a polycarbonate-based polyurethane (CarboSil) that contains blended NO donor, S-nitroso-N-acetylpenicillamine (SNAP). This sample, SNAP-ZnO, continuously sustained NO release above 0.5 × 10-10 mol cm-2 min-1 for 14 days while samples containing only SNAP dropped below physiological levels within 24 h. The ZnO-NP topcoat improved NO release and reduced the amount of SNAP leached by 55% over a 7-day period. ICP-MS data observed negligible Zn ion release into the environment, suggesting longevity of the catalyst within the material. Compared to samples with no NO-release, the SNAP-ZnO films had a 99.03% killing efficacy against Staphylococcus aureus and 87.62% killing efficacy against Pseudomonas aeruginosa. A cell cytotoxicity study using mouse fibroblast 3T3 cells also noted no significant difference in viability between the controls and the SNAP-ZnO material, indicating no toxicity toward mammalian cells. The studies indicate that the synergy of combining a metal ion catalyst with a NO-releasing polymer significantly improved NO-release kinetics and antimicrobial activity for device coating applications. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 00A: 000-000, 2019. |
| Databáze: | OpenAIRE |
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