Clinical performance of a specific granulocyte colony stimulating factor ELISA to determine its concentration in follicular fluid as a predictor of implantation success during in vitro fertilization
| Autor: | K. F. Devreker, Jean-Michel Foidart, Thomas D'Hooghe, Laure Noel, G. Verheyen, Herman Tournaye, S. Perrier d’Hauterive, Michelle Nisolle, Carine Munaut |
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| Přispěvatelé: | Surgical clinical sciences, Biology of the Testis, Centre for Reproductive Medicine - Gynaecology, Vriendenkring VUB |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
030219 obstetrics & reproductive medicine
In vitro fertilisation medicine.diagnostic_test business.industry Endocrinology Diabetes and Metabolism medicine.medical_treatment Clinical performance Obstetrics and Gynecology 030209 endocrinology & metabolism Follicular fluid Granulocyte colony-stimulating factor Andrology 03 medical and health sciences 0302 clinical medicine Endocrinology Human fertilization fertilization Immunoassay Granulocyte colony stimulating factor embryo selection Obstetrics and Gynaecology medicine business in vitro fertilization |
| Popis: | This study aimed to demonstrate the clinical performance of an ultra-sensitive follicular fluid (FF) granulocyte colony stimulating factor (G-CSF) immunoassay to confirm previous work, indicating a correlation between FF G-CSF concentration and live birth potential of the corresponding embryo after in vitro fertilization. This study was a noninterventional, prospective, diagnostic clinical multicentric study conducted between August 2012 and January 2014 with 396 single embryo transfers (SETs) from 278 subjects. During oocyte retrieval, FF was individually collected. Embryo morphology and implantation success were evaluated. The implantation success rate in the high G-CSF group (32.3%) was higher than the overall rate (27.5%). Similarly, for embryos with optimal morphology, implantation success rates were highest among those in the high G-CSF concentration category (34.5%) compared with low (19.6%) and intermediate (29.8%) G-CSF concentration categories. Significant differences in mean G-CSF concentrations were observed between the study sites. To minimize bias, analyses were repeated using data from the center with the largest number of SETs. In alignment with the overall analysis, this center demonstrated a 43% greater probability of implantation for optimal embryos with high G-CSF compared to the general implantation rate among optimal embryos and a 327% increase compared with the implantation rate of optimal embryos with low G-CSF. ispartof: GYNECOLOGICAL ENDOCRINOLOGY vol:36 issue:1 pages:44-48 ispartof: location:England status: published |
| Databáze: | OpenAIRE |
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