Evidence of a diverse T cell receptor repertoire for acetylcholine receptor, the autoantigen of myasthenia gravis
| Autor: | Carlayne E. Jackson, Günter J. Hämmerling, Anthony J. Infante, Ellen Kraig, Raghavanpillai Raju, Jacques Baillargeon, Lisa Lott, Chella S. David |
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| Rok vydání: | 2003 |
| Předmět: |
Adult
Male DNA Complementary Receptors Antigen T-Cell alpha-beta T cell Immunology chemical and pharmacologic phenomena Biology Autoantigens Epitope Epitopes Mice HLA-DR3 Antigen Antigen Myasthenia Gravis medicine Animals Humans Immunology and Allergy Cytotoxic T cell Receptors Cholinergic Receptor Aged Acetylcholine receptor Hybridomas Polymorphism Genetic T-cell receptor hemic and immune systems Middle Aged medicine.disease Myasthenia gravis Disease Models Animal medicine.anatomical_structure Female |
| Zdroj: | Journal of Autoimmunity. 21:167-174 |
| ISSN: | 0896-8411 |
| Popis: | We utilized two methods to look for T cell clonal expansions in myasthenia gravis (MG). We analyzed TCRBV CDR3 length polymorphism (spectratyping) to look for evidence of clonal expansion of CD4 or CD8 T cells directly from peripheral blood of MG patients. No statistically significant differences were found between the diversity of TCR repertoires in MG patients compared to normal control individuals when analyzed as groups. Rare oligoclonal expansions were detected in some individual MG patients but the significance of these findings is unclear. Next, we analyzed a panel of T cell hybridomas from acetylcholine receptor (AChR) immunized, MG-susceptible HLA-DR3 transgenic mice. The epitope specificity, TCRBV gene usage and CDR3 sequences of these hybridomas were highly diverse. We conclude there is only limited evidence for restricted TCR repertoire usage in human MG and suggest this may be due to the inability of HLA-DR molecules to select for restricted TCR recognition of AChR epitopes. |
| Databáze: | OpenAIRE |
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