Protein malnutrition modifies medullary neuronal recruitment in response to intermittent stimulation of the baroreflex
| Autor: | Deoclécio Alves Chianca-Jr, Luciano Gonçalves Fernandes, Míriam Carmo Rodrigues-Barbosa, Fernanda Cacilda dos Santos Silva, Cláudia Martins Carneiro, Lisandra Brandino de Oliveira, Carlos Henrique Xavier |
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| Rok vydání: | 2012 |
| Předmět: |
c-fos
Male medicine.medical_specialty Neuroscience(all) Clinical Neurology Stimulation Blood Pressure Baroreflex c-Fos Phenylephrine Heart Rate Internal medicine Medicine Animals Vasoconstrictor Agents Molecular Biology Medulla Neurons Analysis of Variance Medulla Oblongata biology business.industry General Neuroscience Malnutrition Parasympathetic Rostral ventrolateral medulla Rats Disease Models Animal Endocrinology nervous system Hypothalamus biology.protein Neurology (clinical) Rostral ventromedial medulla business Sympathetic Proto-Oncogene Proteins c-fos medicine.drug Developmental Biology |
| Zdroj: | Brain Research. 1483:20-30 |
| ISSN: | 0006-8993 |
| DOI: | 10.1016/j.brainres.2012.08.019 |
| Popis: | Protein malnutrition after weaning changes the neurotransmission in neural pathways that organize cardiovascular reflexes in rats. The present study evaluates whether protein malnutrition alters the expression of c-fos protein (immediate-early gene expression) in central areas involved in the control of cardiovascular reflexes after intermittent stimulation of the baroreflex. The main nuclei we focused were paraventricular hypothalamus (PVH); nucleus tract solitarii (NTS); rostral ventromedial medulla (RVMM); rostral (RVLM) and caudal ventrolateral medulla (CVLM). Male Fisher rats at 28 days were submitted to two different isocaloric diets during the subsequent 35 days: control (CT) (15% protein) and malnourished (MN) (6% protein). thirty min of intermittent (every 3 min) baroreflex stimulation was performed by infusing phenylephrine (Phe—0.25 mM) or, as control, 0.9% NaCl (Sal). Following ninety minutes, animals were anesthetized and perfused. The removed brains were sectioned (35 μm) and used for c-fos immunohistochemistry. Images were analyzed using the software Leica Q Win. Despite not altering the baseline MAP, malnutrition increased baseline HR and expression of c-fos in RVMM. Increases in c-fos expression after intermittent stimulation of baroreflex were evident in the PVH, medial NTS and CVLM in both dietary protocols. Current data further revealed a differential neuronal recruitment to stimulation of baroreflex in the caudal commissural and rostral NTS and RVLM of MN. We conclude that protein malnutrition modifies the cardiovascular control and the pattern of central response to baroreflex stimulation. |
| Databáze: | OpenAIRE |
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