Grape seed pro‐anthocyanidins ameliorates radiation‐induced lung injury

Autor: Jianming Cai, Wen Liu, Fu Gao, Jin Ni, Feng He, Pei Zhang, Jianguo Cui, Hainan Zhao, Bailong Li, Ying Cheng, Yanyong Yang, Yijuan Huang, Hu Liu
Rok vydání: 2014
Předmět:
Antioxidant
medicine.medical_treatment
Blotting
Western
Radiation-Protective Agents
Biology
Lung injury
epithelial–mesenchymal transition
Real-Time Polymerase Chain Reaction
grape seed pro-anthocyanidins
Immunoenzyme Techniques
Lipid peroxidation
Mice
chemistry.chemical_compound
medicine
Animals
Humans
Vitis
RNA
Messenger
Epithelial–mesenchymal transition
radiation-induced lung injury
Fibroblast
reactive oxygen species
chemistry.chemical_classification
Reactive oxygen species
Plant Extracts
Reverse Transcriptase Polymerase Chain Reaction
Electron Spin Resonance Spectroscopy
Original Articles
Lung Injury
Cell Biology
medicine.disease
Mice
Inbred C57BL
Radiation Injuries
Experimental
medicine.anatomical_structure
Radiation-induced lung injury
chemistry
Gamma Rays
Apoptosis
Seeds
Immunology
Cancer research
Cytokines
Molecular Medicine
Female
Zdroj: Journal of Cellular and Molecular Medicine
ISSN: 1582-4934
1582-1838
DOI: 10.1111/jcmm.12276
Popis: Radiation-induced lung injury (RILI) is a potentially fatal and dose-limiting complication of thoracic radiotherapy. This study was to investigate the protective effects of grape seed pro-anthocyanidins (GSPs), an efficient antioxidant and anti-carcinogenic agent, on RILI. In our study, it was demonstrated that acute and late RILI was ameliorated after GSPs treatment possibly through suppressing TGF-β1/Smad3/Snail signalling pathway and modulating the levels of cytokines (interferon-γ, IL-4 and IL-13) derived from Th1/Th2 cells. In addition, a sustained high level of PGE2 was also maintained by GSPs treatment to limited fibroblast functions. As shown by electron spin resonance spectrometry, GSPs could scavenge hydroxyl radical (•OH) in a dose-dependent manner, which might account for the mitigation of lipid peroxidation and consequent apoptosis of lung cells. In vitro, GSPs radiosensitized lung cancer cell A549 while mitigating radiation injury on normal alveolar epithelial cell RLE-6TN. In conclusion, the results showed that GSPs protects mice from RILI through scavenging free radicals and modulating RILI-associated cytokines, suggesting GSPs as a novel protective agent in RILI.
Databáze: OpenAIRE
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