Identification of the resistance of a novel molecule heat shock protein 90α (HSP90α) in Microtus fortis to Schistosoma japonicum infection
| Autor: | Zhi-qiang Qin, Yuan-Jing Yu, De-Hui Xiong, Wei-Xin Hu, Gang Cheng, Qing-Ren Zeng, Qiang Gong |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Veterinary (miscellaneous) Genetic Vectors Schistosoma japonicum Animals Genetically Modified Rodent Diseases Mice Transduction Genetic In vivo Complementary DNA Heat shock protein parasitic diseases Animals HSP90 Heat-Shock Proteins Parasite Egg Count Gene Library Mice Inbred BALB C Expression vector Schistosoma Japonicum Infection biology Arvicolinae biology.organism_classification Survival Analysis Molecular biology Immunity Innate Microtus fortis Retroviridae Infectious Diseases Liver Culture Media Conditioned Schistosomiasis japonica Insect Science Immunology Expression cloning Parasitology |
| Zdroj: | Acta Tropica. 115:220-226 |
| ISSN: | 0001-706X |
| Popis: | Microtus fortis is a naturally resistant vertebrate host of Schistosoma japonicum by preventing completion of parasite's life cycle. Sera of M. fortis were found to have anti-schistosome effect in vitro and in vivo. In order to identify genes associated with the anti-schistosome effect of M. fortis, we screened a M. fortis marrow cDNA expression library by expression cloning and identified a 331-bp clone gC14.75. It was the homologue of heat shock protein 90alpha (HSP90alpha). Full-length of M. fortis HSP90alpha gene, Mf-HSP90alpha, was amplified according to gC14.75 and Cricetulus griseus HSP90alpha. To test the potential anti-schistosome function of Mf-HSP90alpha, we prepared conditioned medium of Mf-HSP90alpha and added it to schistosomula cultured in vitro. It caused 27.0% schistosomula death rate in 96h, which was considerably higher than that of negative control. We transferred Mf-HSP90alpha by retroviral expression vector pLXSN into mice to investigate its anti-schistosome effect in vivo. Compared with those of DMEM injection control, mice injected with Mf-HSP90alpha recombinant retrovirus had 40.8% worm burden reduction and 57.9% reduction in liver eggs per gram (LEPG) indicating its anti-schistosome effect in vivo. Taken together, our results suggested Mf-HSP90alpha as a novel anti-schistosome molecule in vitro and in vivo. |
| Databáze: | OpenAIRE |
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