Differential effects of peripheral and transitional prostatic stromal cells on tumorigenesis
| Autor: | Zhong Wang, Bao Li, Juan Zhou, Yu-Bing Peng, Ming Zhang, Qi Chen, Jun Da, Yan Gao, Wen-Ji Li, Hao Wang |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Adult
Male CDC25A DNA Complementary Stromal cell Carcinogenesis Mice Nude TACSTD2 Biology Real-Time Polymerase Chain Reaction medicine.disease_cause Mice Young Adult Prostate cancer DU145 Antigens Neoplasm medicine Animals Humans cdc25 Phosphatases Cells Cultured DNA Primers Base Sequence Reverse Transcriptase Polymerase Chain Reaction Microarray analysis techniques Prostate Prostatic Neoplasms medicine.disease Peripheral Cancer research Stromal Cells Cell Adhesion Molecules |
| Zdroj: | Frontiers in Bioscience. 20:716-727 |
| ISSN: | 1093-4715 1093-9946 |
| DOI: | 10.2741/4332 |
| Popis: | The human prostate contains two types of stromal cells, peripheral stromal cells (PSCs) and transitional stromal cells (TSCs). Here, we demonstrate the effects of PSCs and TSCs on tumorigenesis in prostate cancer (PCa) and identify the mechanisms underlying these effects. Using microarray analysis, we identified 3,643 differentially expressed genes in cocultures of TSCs, PSCs, and DU145 cells, a human prostate cancer cell line. Expression of cell division cycle 25 homolog A (CDC25A) was lower and that of tumor-associated calcium signal transducer 2 (TACSTD2) was higher in TSCs than in PSCs. Additionally, increased CDC25A expression or decreased TACSTD2 expression modulated the survival, growth, and migration of DU145 cells. These data suggest that PSCs promote and TSCs inhibit tumorigenesis by regulating the expression of CDC25A and TACSTD2. |
| Databáze: | OpenAIRE |
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