Electroaddressed immobilization of recombinant HIV-1 P24 capsid protein onto screen-printed arrays for serological testing
| Autor: | Christophe A. Marquette, Bernard Mandrand, Carole Chaix, François Mallet, Emmanuelle Imbert-Laurenceau, Loïc J. Blum |
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| Rok vydání: | 2005 |
| Předmět: |
P24 capsid protein
Serial dilution Molecular Sequence Data HIV Core Protein p24 Biophysics HIV Antibodies Polypyrrole Biochemistry law.invention chemistry.chemical_compound law HIV Seronegativity HIV Seropositivity Humans Serologic Tests Amino Acid Sequence Biochip Electrodes Molecular Biology Chemiluminescence Reproducibility Chromatography Molecular Structure biology Chemistry Immune Sera Reproducibility of Results Cell Biology Molecular biology Recombinant Proteins Kinetics Capsid Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization biology.protein Recombinant DNA Sequence Alignment |
| Zdroj: | Analytical Biochemistry. 340:14-23 |
| ISSN: | 0003-2697 |
| Popis: | A serological chemiluminescent biochip was designed based on screen-printed electrode arrays composed of nine 1-mm2 electrodes. Arrays were shown to be produced with good batch-to-batch reproducibility (standard deviations of 4.4 and 12.0% for ferricyanide oxidation potential and current, respectively) and very good reproducibility within a particular array (2.0 and 7.5% standard deviations for the same controls). Electrode arrays were used to electroaddress various bioconjugate structures comprising a recombinant HIV-1 P24 capsid protein (RH24K) in polypyrrole film. Entrapment of RH24K preimmobilized onto maleic anhydride-alt-methyl vinyl ether copolymer was shown to be the more efficient immobilization procedure. This addressed sensing layer enabled the detection of anti-P24 antibodies at a concentration of 3.5 ng/ml through peroxidase-labeled anti-human immunoglobulin G reaction. The biochip was used to perform an HIV-1 serological test in human sera. HIV-1 seropositive and seronegative sera were easily discriminated using serum dilutions greater than 1/10,000. |
| Databáze: | OpenAIRE |
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