Cytoprotective mechanism of ferulic acid against high glucose-induced oxidative stress in cardiomyocytes and hepatocytes

Autor:	Yong He, Weibin Bai, Jianxia Sun, Shiyi Ou, Luona Wen, Rui Jiao, Yuan Song, Yunfeng Hu, Xichun Peng
Přispěvatelé:	National Natural Science Foundation of China, First Affiliated Hospital of Jinan University, Science and Technology Council of Guangdong
Jazyk:	angličtina
Rok vydání:	2016
Předmět:	0301 basic medicine medicine.medical_specialty cardiomyocytes lcsh:TX341-641 medicine.disease_cause Ferulic acid 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine medicine oxidative stress Viability assay Food Related Nutrition Nutrition and Dietetics Public Health Environmental and Occupational Health Glutathione Phenolic acid Molecular nutrition ferulic acid hepatocytes 030104 developmental biology Endocrinology L-Glucose chemistry Apoptosis 030220 oncology & carcinogenesis Original Article Signal transduction lcsh:Nutrition. Foods and food supply Oxidative stress Food Science
Zdroj:	Food & Nutrition Research, Vol 60, Iss 0, Pp 1-9 (2016) Food & Nutrition Research; Vol 60 (2016) Food & Nutrition Research
ISSN:	1654-661X 1654-6628
Popis:	Background : Ferulic acid (FA), a phenolic acid, is a potential therapy for diabetes mellitus. FA has been shown to protect against hepatic and myocardial injury and oxidative stress in obese rats with late-stage diabetes, but the mechanism of the antioxidative activity of FA is still unclear. Objective : The aim of this study was to elucidate whether FA can prevent damage to cardiomyocytes and hepatocytes caused by high glucose (HG)-induced oxidative stress and whether the protection effects of FA on these cells are related to the Keap1-Nrf2-ARE signaling pathways. Design : Cells were divided into four groups: a control group (cultured with normal medium), an HG group (medium containing 80 mmol/L glucose), an FA+HG group (medium containing 80 mmol/L glucose and 1, 5, or 10 µg/mL FA), and a dimethylbiguanide (DMBG)+HG group (medium containing 80 mmol/L glucose and 50 µg/mL DMBG). Results : FA treatment significantly increased cell viability and significantly decreased cell apoptosis compared with the HG-treated group. Moreover, FA down-regulated the expression of Keap1 protein and up-regulated the expression of Nrf2 protein and gene transcription of HO-1 and glutathione S-transferase (GST) in a dose-dependent manner. Conclusion : FA alleviated the HG-induced oxidative stress and decreased cell apoptosis in hepatocytes and cardiomyocytes. These effects were associated with the Keap1-Nrf2-ARE signaling pathway. Keywords: ferulic acid; oxidative stress; cardiomyocytes; hepatocytes (Published: 10 February 2016) Citation: Food & Nutrition Research 2016, 60: 30323 - http://dx.doi.org/10.3402/fnr.v60.30323
Databáze:	OpenAIRE
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