Multiple evidences suggest sox2 as the main driver of a young and complex sex determining ZW/ZZ system in turbot (Scophthalmus maximus)
| Autor: | Francesc Piferrer, Blanca Álvarez-Blázquez, Santiago Cabaleiro, Carmen Bouza, Xoana Taboada, Ana Viñas, Antonio Gómez-Tato, Diego Robledo, Ana Blanco, Paulino Martínez |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0303 health sciences
Classical genetics zoology sex determination Chromosome Genomics Single-nucleotide polymorphism Genome-wide association study Biology Turbot biology.organism_classification Genetic architecture Scophthalmus 03 medical and health sciences Sox 2 0302 clinical medicine Evolutionary biology Genotype evolution 030217 neurology & neurosurgery 030304 developmental biology |
| Zdroj: | e-IEO. Repositorio Institucional Digital de Acceso Abierto del Instituto Español de Oceanografía instname |
| DOI: | 10.1101/834556 |
| Popis: | A major challenge in evolutionary biology is to find an explanation for the variation in sex-determining (SD) systems across taxa and to understand the mechanisms driving sex chromosome differentiation. We studied the turbot, holding a ZW/ZZ SD system and no sex chromosome heteromorphism, by combining classical genetics and genomics approaches to disentangle the genetic architecture of this trait. RAD-Seq was used to genotype 18,214 SNPs on 1,135 fish from 36 families and a genome wide association study (GWAS) identified a ∼ 6 Mb region on LG5 associated with sex (P < 0.05). The most significant associated markers were located close to sox2, dnajc19 and fxr1 genes. A segregation analysis enabled narrowing down the associated region and evidenced recombination suppression in a region overlapping the candidate genes. A Nanopore/Illumina assembly of the SD region using ZZ and WW individuals identified a single SNP fully associated with Z and W chromosomes. RNA-seq from 5-90 day-old fish detected the expression along the gonad differentiation period of a short non-coding splicing variant (ncRNA) included in a vertebrate-conserved long non-coding RNA overlapping sox2. qPCR showed that sox2 was the only differentially expressed gene between males and females at 50-55 days post fertilization, just prior the beginning of gonad differentiation. More refined information on the involvement of secondary genetic and environmental factors and their interactions on SD was gathered after the analysis of a broad sample of families. Our results confirm the complex nature of SD in turbot and support sox2 as its main driver. |
| Databáze: | OpenAIRE |
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