Tau expression in model adenocarcinomas correlates with docetaxel sensitivity in tumour-bearing mice
| Autor: | M. C. Bissery, R. Veitia, C. Martinez, A. Fellous |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Cancer Research
medicine.medical_specialty Paclitaxel Microtubule-associated protein Tau protein Immunoblotting Mice Nude Breast Neoplasms tau Proteins Docetaxel Adenocarcinoma Polymerase Chain Reaction Mice Western blot Tubulin Internal medicine medicine Neoplasm Animals Humans RNA Messenger Mice Inbred C3H biology medicine.diagnostic_test Carcinoma Mammary Neoplasms Experimental medicine.disease Antineoplastic Agents Phytogenic Neoplasm Proteins Mice Inbred C57BL Pancreatic Neoplasms Endocrinology medicine.anatomical_structure Oncology Mechanism of action Drug Resistance Neoplasm biology.protein Cancer research Female Taxoids medicine.symptom Pancreas Microtubule-Associated Proteins medicine.drug Research Article |
| Zdroj: | British Journal of Cancer |
| ISSN: | 0007-0920 |
| Popis: | Docetaxel is a new taxoid with clinical activity in breast and lung cancer. Using docetaxel-sensitive and -refractory mammary and pancreatic murine tumours, as well as human-derived neoplasms, we investigated if a determinant of docetaxel sensitivity could be found at the level of its mechanism of action. Because microtubules represent the cellular targets of the drug, we studied their heterogeneity in the tumour models to try to explain the differences in drug sensitivity. Reverse transcription-polymerase chain reaction (RT-PCR) analysis of the expression of microtubular components showed that levels of Mbeta4-tubulin and Tau mRNAs were higher in the murine sensitive neoplasms than in the refractory ones. It was also found that Tau protein levels differed markedly among the tumours. In the human-derived sensitive neoplasm, beta-tubulins and some Tau isoforms were found to be more abundant than in the resistant one. Western blot analysis of MAP2 revealed the presence of several immunoreactive species. Some of these polypeptides were also found in higher amounts in the docetaxel-sensitive tumours. The possible meaning of these correlations is discussed in connection with the regulation of microtubule dynamics. Images Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 |
| Databáze: | OpenAIRE |
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