Cloning and characterization of cDNA encoding zebrafish Danio rerio NM23-B gene

Autor:	Seung-Hwi Lee, Jae-Seong Lee
Rok vydání:	2000
Předmět:	DNA Complementary animal structures Ultraviolet Rays Molecular Sequence Data Oligonucleotides Danio DNA Single-Stranded Gene Expression Complementary DNA Genetics Animals Tissue Distribution Amino Acid Sequence Cloning Molecular Gene Zebrafish Monomeric GTP-Binding Proteins Repetitive Sequences Nucleic Acid Cloning chemistry.chemical_classification Messenger RNA Base Sequence Sequence Homology Amino Acid biology Sequence Analysis DNA General Medicine NM23 Nucleoside Diphosphate Kinases Telomere Blotting Northern biology.organism_classification Molecular biology Amino acid Open reading frame Cross-Linking Reagents chemistry Nucleoside-Diphosphate Kinase RNA Female Sequence Alignment Protein Binding Transcription Factors
Zdroj:	Gene. 245:75-79
ISSN:	0378-1119
DOI:	10.1016/s0378-1119(00)00037-8
Popis:	A full-length zebrafish NM23-B cDNA was cloned and sequenced. The zebrafish NM23-B cDNA consists of 624 bp with an open reading frame of 153 amino acids. NM23-B mRNA of approximately 0.7 kb is present in adult zebrafish tissues. Zebrafish NM23-B his-tagged protein (17 kDa) was produced in E. coli and characterized by binding and UV-cross-linking to a single-stranded telomeric repeat (TTAGGG) 6 . This is the first report to show that fish have a NM23-H2 homologue that is similar to that in humans.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b6091146829c7d999e0533a7e0a81135 https://doi.org/10.1016/s0378-1119(00)00037-8 Zobrazit plný text záznamu Full Text from ScienceDirect