Comprehensive phenotyping reveals interactions and functions of Arabidopsis thaliana TCP genes in yield determination

Autor: Richard G. H. Immink, Elwin B. van der Auweraert, Sam W. van Es, Sylvia Rodrigues da Silveira, Gerco C. Angenent, Aalt D. J. van Dijk
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0106 biological sciences
0301 basic medicine
plant architecture
phenotyping
Arabidopsis thaliana
Vegetative reproduction
Bioinformatics
Mutant
Arabidopsis
Plant Science
Computational biology
Biology
01 natural sciences
Wiskundige en Statistische Methoden - Biometris
Rosette (botany)
03 medical and health sciences
BIOS Applied Bioinformatics
Bioinformatica
Genetics
Laboratorium voor Moleculaire Biologie
BIOS Plant Development Systems
Photosynthesis
Enhancer
Onderzoekschool EPS
Gene
Transcription factor
Mathematical and Statistical Methods - Biometris
2. Zero hunger
TCP transcription factor
Arabidopsis Proteins
fungi
food and beverages
Original Articles
Cell Biology
plant growth
biology.organism_classification
yield
Phenotype
030104 developmental biology
EPS Graduate School
Mutagenesis
Site-Directed
Genetic redundancy
Original Article
Laboratory of Molecular Biology
EPS
Transcription Factors
010606 plant biology & botany
Zdroj: The Plant Journal, 99(2), 316-328
The Plant Journal
The Plant Journal 99 (2019) 2
ISSN: 0960-7412
Popis: Summary Members of the Arabidopsis thaliana TCP transcription factor (TF) family affect plant growth and development. We systematically quantified the effect of mutagenizing single or multiple TCP TFs and how altered vegetative growth or branching influences final seed yield. We monitored rosette growth over time and branching patterns and seed yield characteristics at the end of the lifecycle. Subsequently, an approach was developed to disentangle vegetative growth and to determine possible effects on seed yield. Analysis of growth parameters showed all investigated tcp mutants to be affected in certain growth aspects compared with wild‐type plants, highlighting the importance of TCP TFs in plant development. Furthermore, we found evidence that all class II TCPs are involved in axillary branch outgrowth, either as inhibitors (BRANCHED‐like genes) or enhancers (JAW‐ and TCP5‐like genes). Comprehensive phenotyping of plants mutant for single or multiple TCP TFs reveals that the proposed opposite functions of class I and class II TCPs in plant growth needs revision and shows complex interactions between closely related TCP genes instead of full genetic redundancy. In various instances, the alterations in vegetative growth or in branching patterns result into negative trade‐off effects on seed yield that were missed in previous studies, showing the importance of comprehensive and quantitative phenotyping.
Significance Statement This study shows the importance of the TCP transcription factor family during Arabidopsis growth and development, coupled to yield and plant architecture. It was found that all class II TCPs are involved in axillary bud outgrowth and that the proposed opposite functioning of class I and class II TCPs requires revision.
Databáze: OpenAIRE
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