Isolation and characterisation of novel phages infecting Lactobacillus plantarum and proposal of a new genus, 'Silenusvirus'
| Autor: | Athanasios Zervas, Alexander Byth Carstens, Martin Hansen, Lea Ellegaard-Jensen, Lars Hestbjerg Hansen, Ifigeneia Kyrkou, Charles M. A. P. Franz, Horst Neve, Witold Kot, Amaru Miranda Djurhuus |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
viruses Denmark 030106 microbiology BACTERIOPHAGES PROTEIN lcsh:Medicine Wine Genome Viral Viral Plaque Assay Biology Proteomics SEQUENCE Genome CLASSIFICATION Article Siphoviridae 03 medical and health sciences Bacteriolysis Species Specificity Phylogenetics Lactobacillus Bacteriophages lcsh:Science Phylogeny 030304 developmental biology Comparative genomics Genetics 0303 health sciences Multidisciplinary 030306 microbiology FRAMESHIFT lcsh:R food and beverages Isolation (microbiology) biology.organism_classification Microscopy Electron Waste Disposal Facilities 030104 developmental biology DNA Viral lcsh:Q Adsorption Bacteria Lactobacillus plantarum |
| Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-10 (2020) Kyrkou, I, Carstens, A B, Ellegaard-Jensen, L, Kot, W, Zervas, A, Djurhuus, A M, Neve, H, Franz, C M A P, Hansen, M & Hansen, L H 2020, ' Isolation and characterisation of novel phages infecting Lactobacillus plantarum and proposal of a new genus, "Silenusvirus" ', Scientific Reports, vol. 10, no. 1, 8763 . https://doi.org/10.1038/s41598-020-65366-6 Kyrkou, I, Carstens, A B, Ellegaard-Jensen, L, Kot, W, Zervas, A, Djurhuus, A M, Neve, H, Franz, C M A P, Hansen, M & Hansen, L H 2020, ' Isolation and characterisation of novel phages infecting Lactobacillus plantarum and proposal of a new genus, “Silenusvirus” ', Scientific Reports, vol. 10, no. 1, 8763 . https://doi.org/10.1038/s41598-020-65366-6 bioRxiv |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-020-65366-6 |
| Popis: | Bacteria of Lactobacillus sp. are very useful to humans. However, the biology and genomic diversity of their (bacterio)phage enemies remains understudied. Knowledge on Lactobacillus phage diversity should broaden to develop efficient phage control strategies. To this end, organic waste samples were screened for phages against two wine-related Lactobacillus plantarum strains. Isolates were shotgun sequenced and compared against the phage database and each other by phylogenetics and comparative genomics. The new isolates had only three distant relatives from the database, but displayed a high overall degree of genomic similarity amongst them. The latter allowed for the use of one isolate as a representative to conduct transmission electron microscopy and structural protein sequencing, and to study phage adsorption and growth kinetics. The microscopy and proteomics tests confirmed the observed diversity of the new isolates and supported their classification to the family Siphoviridae and the proposal of the new phage genus “Silenusvirus”. |
| Databáze: | OpenAIRE |
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