Peroxiredoxin V Reduces β-Lapachone-induced Apoptosis of Colon Cancer Cells
| Autor: | Hu-Nan Sun, Dong Kee Jeong, Taeho Kwon, Mei-Hua Jin, Dan-Ping Xie, Yi-Xi Gong, Dong-Sun Lee, Ji-Su Kim, Ying-Hua Jin, Gui-Nan Shen, Ying-Hao Han, Nisansala Chandimali, Yue Liu, Yu-Dong Cui |
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| Rok vydání: | 2019 |
| Předmět: |
Cancer Research
medicine.diagnostic_test Colon Chemistry Wnt signaling pathway Apoptosis Peroxiredoxins General Medicine Flow cytometry Cell biology Blot Oncology Annexin Cell Line Tumor Colonic Neoplasms medicine Humans MTT assay Signal transduction Reactive Oxygen Species Peroxiredoxin Wnt Signaling Pathway Naphthoquinones |
| Zdroj: | Anticancer Research. 39:3677-3686 |
| ISSN: | 1791-7530 0250-7005 |
| Popis: | Background/aim Peroxiredoxin (Prx) V has been known as an antioxidant enzyme which scavenges intracellular reactive oxygen species (ROS). Also, Prx V has been shown to mediate cell apoptosis in various cancers. However, the mechanism of Prx V-induced apoptosis in colon cancer cells remains unknown. Thus, in this study we analyzed the effects of Prx V in β-lapachone-induced apoptosis in SW480 human colon cancer cells. Materials and methods β-lapachone-induced apoptosis was analyzed by the MTT assay, western blotting, fluorescence microscopy, Annexin V staining and flow cytometry. Results Overexpression of Prx V, significantly decreased β-lapachone-induced cellular apoptosis and Prx V silencing increased β-lapachone-induced cellular apoptosis via modulating ROS scavenging activity compared to mock SW480 cells. In addition, to further explore the mechanism of Prx V regulated β-lapachone-induced SW480 cells apoptosis, the Wnt/β-catenin signaling was studied. The Wnt/ β-catenin signaling pathway was found to be induced by β-lapachone. Conclusion Prx V regulates SW480 cell apoptosis via scavenging ROS cellular levels and mediating the Wnt/β-catenin signaling pathway, which was induced by β-lapachone. |
| Databáze: | OpenAIRE |
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