Different expression of B7-H3 in the caput, corpus, and cauda of the epididymis in mouse
| Autor: | Jianquan Hou, Guangbo Zhang, Hexing Yuan, Xuedong Wei, Xuefeng Zhang, Chenhao Zhou, Miao Li, Kai Li |
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| Rok vydání: | 2017 |
| Předmět: |
Male
endocrine system B7 Antigens Mouse Urology Cauda 030232 urology & nephrology Corpus lcsh:RC870-923 Andrology Mice 03 medical and health sciences 0302 clinical medicine medicine Animals Epididymis Sperm maturation Caput urogenital system business.industry Ig superfamily General Medicine lcsh:Diseases of the genitourinary system. Urology Sperm Mice Inbred C57BL medicine.anatomical_structure B7-H3 Reproductive Medicine 030220 oncology & carcinogenesis business Research Article |
| Zdroj: | BMC Urology, Vol 17, Iss 1, Pp 1-5 (2017) BMC Urology |
| ISSN: | 1471-2490 |
| DOI: | 10.1186/s12894-017-0215-5 |
| Popis: | Background B7-H3, a member of the B7 family of the Ig superfamily of proteins, has been detected in the epididymis, which is a storage organ related to sperm maturation. However, the characteristics of its expression in different regions of the epididymis remain unknown. Our aim was to investigate the expression of B7-H3 in the caput, corpus, and cauda of the epididymis. Methods We extracted epididymis specimens from adult male C57BL/6 mice. The expression of B7-H3 was then measured with immunohistochemistry, enzyme-linked immunosorbent assay (ELISA) and western blotting. Results B7-H3 protein was predominantly detected on the membrane and in the cytoplasm of the principal cells in the epididymis. Moreover, the level of B7-H3 in the corpus of the mouse epididymis was significantly higher than that in the caput (p 0.05). Conclusions The caput, corpus, and cauda of the mouse epididymis all expressed B7-H3 protein. However, the levels of B7-H3 were different in the three regions of the epididymis. |
| Databáze: | OpenAIRE |
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