Expression of P-glycoprotein, encoded by MDR 1 gene, a metabolically active efflux pump in murine mast cells
| Autor: | David Wein, Sudhir Gupta |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Antimetabolites
Antineoplastic Cancer Research Blotting Western Molecular Sequence Data Polymerase Chain Reaction Rhodamine 123 Flow cytometry Mice chemistry.chemical_compound Western blot Cyclosporin a medicine Animals ATP Binding Cassette Transporter Subfamily B Member 1 Mast Cells P-glycoprotein Base Sequence biology medicine.diagnostic_test Rhodamines Mast cell Molecular biology Drug Resistance Multiple Blot Interleukin 33 medicine.anatomical_structure Oncology chemistry Cyclosporine biology.protein RNA |
| Zdroj: | Cancer Letters. 101:241-246 |
| ISSN: | 0304-3835 |
| DOI: | 10.1016/0304-3835(96)04146-8 |
| Popis: | Mast cell line (MC/9) derived from normal murine liver was examined for the expression of P-glycoprotein (P-gp) at the level of protein with C-219 and JSB-1 monoclonal antibodies, using flow cytometry and Western blot, and at the level of mRNA by the reverse transcriptase-polymerase chain reaction. The function of P-gp was analyzed by the accumulation and efflux of rhodamine 123 (Rh123) in the presence or absence of cyclosporin A (CSA) and a non-immunosuppressive analog of CSA (CSA-1). P-gp both at the protein and mRNA levels was expressed in mast cells. Intracellular accumulation of Rh123, in the presence of CSA and CSA-1 was significantly greater than in their absence. Furthermore, both CSA and CSA-1 inhibited Rh123 efflux from mast cells. These data suggest the presence of a functionally active P-gp in mast cells. A possible physiologic role for P-gp in the secretion of certain mediators/cytokines from mast cells is suggested. |
| Databáze: | OpenAIRE |
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