Population Genetic Analysis of the Theileria annulata Parasites Identified Limited Diversity and Multiplicity of Infection in the Vaccine From India
| Autor: | Pankaj Kumar, Jaspreet Singh Arora, Satparkash Singh, Vandna Bhanot, Paresh Sharma, Vasundhra Bhandari, Madhumanti Barman, Sonti Roy |
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| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Veterinary medicine 030231 tropical medicine Population lcsh:QR1-502 Population genetics Microbiology lcsh:Microbiology 03 medical and health sciences 0302 clinical medicine parasitic diseases Genetic variation Genotype Theileria Parasite hosting education Genotyping Original Research 030304 developmental biology 0303 health sciences education.field_of_study Genetic diversity biology schizont stage vaccine population genetics genetic diversity biology.organism_classification Theileria annulata genotyping |
| Zdroj: | Frontiers in Microbiology Frontiers in Microbiology, Vol 11 (2021) |
| ISSN: | 1664-302X |
| Popis: | Background:Apicomplexan parasiteTheileria annulatacauses significant economic loss to the livestock industry in India and other tropical countries. In India, parasite control is mainly dependent on the live attenuated schizont vaccine and the drug buparvaquone. For effective disease control, it is essential to study the population structure and genetic diversity of theTheileria annulatafield isolates and vaccine currently used in India.Methodology/Results:A total of 125T. annulataisolates were genotyped using 10 microsatellite markers from four states belonging to different geographical locations of India. Limited genetic diversity was observed in the vaccine isolates when compared to the parasites in the field; a level of geographical substructuring was evident in India. The number of genotypes observed per infection was highest in India when compared to other endemic countries, suggesting high transmission intensity and abundance of ticks in the country. A reduced panel of four markers can be used for future studies in these for surveillance of theT. annulataparasites in India.Conclusion:High genetic variation between the parasite populations in the country suggests their successful spread in the field and could hamper the disease control programs. Our findings provide the baseline data for the diversity and population structure ofT. annulataparasites from India. The low diversity in the vaccine advocates improving the current vaccine, possibly by increasing its heterozygosity. The reduced panel of the markers identified in this study will be helpful in monitoring parasite and its reintroduction afterTheileriaeradication. |
| Databáze: | OpenAIRE |
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