Direct measurement of pregnanediol 3-glucuronide (PDG) in dried urine spots by liquid chromatography-mass spectrometry to detect ovulation
| Autor: | Belinda Whittle, Reena Desai, Rama Nimmagadda, Timothy D. Handelsman, David J. Handelsman, Richard A. Anderson, Karolina Skorupskaite |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Ovulation Endocrinology Diabetes and Metabolism media_common.quotation_subject Clinical Biochemistry Urine Luteal phase Urinalysis Biochemistry Mass Spectrometry Excretion 03 medical and health sciences 0302 clinical medicine Endocrinology Liquid chromatography–mass spectrometry Follicular phase medicine Humans Molecular Biology Menstrual cycle Menstrual Cycle media_common Ovulation Detection Chromatography medicine.diagnostic_test Chemistry Cell Biology 030104 developmental biology 030220 oncology & carcinogenesis Immunoassay Molecular Medicine Pregnanediol Female Biomarkers Chromatography Liquid |
| Zdroj: | Handelsman, D J, Nimmagadda, R, Desai, R, Handelsman, T D, Whittle, B, Skorupskaite, K & Anderson, R A 2021, ' Direct Measurement of Pregnanediol 3-Glucuronide (PDG) In Dried Urine Spots by Liquid Chromatography-Mass Spectrometry to Detect Ovulation ', Journal of Steroid Biochemistry and Molecular Biology . https://doi.org/10.1016/j.jsbmb.2021.105900 |
| ISSN: | 1879-1220 |
| DOI: | 10.1016/j.jsbmb.2021.105900 |
| Popis: | Background Non-invasive self-testing using an objective chemical method to detect ovulation is valuable for women planning conception, practising contraception or undergoing infertility investigations or treatment. Methods Based on luteal phase secretion of progesterone (P4) and excretion of its major metabolite, pregnanediol glucuronide (PDG), we developed a novel direct liquid chromatography-mass spectrometry (LCMS) method to measure PDG and other steroid glucuronides in urine and in dried urine spots (DUS) without deconjugation or derivatization. Urine PDG by LCMS and immunoassay (P3G) and P4 by immunoassay with and without adjustment for creatinine were evaluated in daily first void urine samples from 10 women through a single menstrual cycle in which ovulation was confirmed by serial transvaginal ultrasound. Results Urine PDG with and without creatinine adjustment was stable during the follicular phase with the expected striking rise in the luteal phase peaking at 5 days after ovulation. Using a single spot urine sample (100 μL) or a DUS ( Conclusions This method holds promise as a non-invasive self-test method for women to determine by an objective chemical method their ovulatory status. |
| Databáze: | OpenAIRE |
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