Limiting dilution analysis of the allo-MHC anti-paternal cytotoxic T cell response II: recurrent spontaneous abortion and the effect of immunotherapy
Autor: | C. E. M. Sharrock, J. M. Pearce, I. T. Manyonda, R. S. Pereira |
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Rok vydání: | 1993 |
Předmět: |
Male
Abortion Habitual Isoantigens Adoptive cell transfer medicine.medical_treatment Immunology Human leukocyte antigen Major histocompatibility complex Major Histocompatibility Complex Pregnancy Recurrence Humans Immunology and Allergy Medicine Cytotoxic T cell Lymphocytes biology business.industry Histocompatibility Testing Immunotherapy T lymphocyte Cytotoxicity Tests Immunologic Kinetics Immunization Immunologic Techniques biology.protein Female Lymphocyte Culture Test Mixed Antibody business Research Article T-Lymphocytes Cytotoxic |
Zdroj: | Clinical and Experimental Immunology. 93:132-137 |
ISSN: | 1365-2249 0009-9104 |
DOI: | 10.1111/j.1365-2249.1993.tb06509.x |
Popis: | SUMMARY Using limiting dilution analysis (LDA) we determined anti-paternal cytotoxic T lymphocyte precursor (CTLp) frequencies in the peripheral blood of 10 women with unexplained recurrent spontaneous abortion (RSA) before and after immunization with paternal lymphocytes. The women and their partners were HLA tissue-typed and none of the women had anti-paternal cytotoxic antibodies (APCA) before immunization. All other known causes of RSA were excluded. All 10 women were found to have high frequencies of specific anti-paternal cytotoxic T cells before immunization (range I 1/1030 to 1/9574). Splitwell analysis showed that these cytotoxic cells were specific to paternal MHC antigens. These frequencies rose significantly following immunization (range 1/683 to 1/4652). The cytotoxic T lymphocyte frequencies against an HLA-mismatched third party varied from woman to woman, but were not affected by the immunization. The LDA data conformed lo single-hit kinetics, indicating that only cytotoxic T ceils were limiting in the assay. Our data are in sharp contrast to the previously held view that women with RSA may be hyporesponsive to paternal MHC antigens. Immunizing such women with paternal leucocytes further sensitizes them. These findings cannot be reconciled with a favourable outcome in the treatment of RSA with immunotherapy. We would argue that this treatment is al best of unproven value, and may even be harmful. Thai these women may sometimes have successful pregnancies following immunotherapy testifies to the effectiveness of the classical MHC antigen-deficient trophoblast as an immunological barrier between mother and fetus. |
Databáze: | OpenAIRE |
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