Modulation of Adipose Tissue Expression of Murine Matrix Metalloproteinases and Their Tissue Inhibitors With Obesity
| Autor: | Alain Colige, H. Roger Lijnen, Desire Collen, Carine Munaut, E Maquoi |
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| Rok vydání: | 2002 |
| Předmět: |
Male
medicine.medical_specialty Transcription Genetic Endocrinology Diabetes and Metabolism Cellular differentiation Adipose tissue macrophages Adipose tissue Biology Matrix metalloproteinase 3T3 cells Mice chemistry.chemical_compound Internal medicine Adipocyte Adipocytes Internal Medicine medicine Animals Zymography Obesity RNA Messenger Enzyme Inhibitors Lung Epididymis Reverse Transcriptase Polymerase Chain Reaction Cell Differentiation Tissue Inhibitor of Metalloproteinases 3T3 Cells Matrix Metalloproteinases Diet medicine.anatomical_structure Endocrinology Adipose Tissue Gene Expression Regulation chemistry Adipogenesis |
| Zdroj: | Diabetes. 51:1093-1101 |
| ISSN: | 1939-327X 0012-1797 |
| Popis: | The potential role of the matrix metalloproteinase (MMP) system in the pathophysiology of the adipose tissue was investigated in a mouse model of nutritionally induced obesity. mRNA levels of 16 MMPs and 4 tissue inhibitors of MMPs (TIMPs) were measured by semiquantitative RT-PCR in adipose tissue isolated from mice maintained for 15 weeks on a standard or high-fat diet. In mice on standard diet, with the exception of MMP-8, all MMP and TIMP transcripts were detected in both gonadal and subcutaneous depots. In obese mice, the expression of MMP-3, -11, -12, -13, and -14 and TIMP-1 mRNAs was upregulated, whereas that of MMP-7, -9, -16, and -24 and TIMP-4 was downregulated. Most MMP and TIMP mRNAs were expressed at higher levels in stromal-vascular cells than in mature adipocytes. Analysis of adipose tissue by in situ fluorescent zymography revealed MMP-dependent proteolytic activities, demonstrating the presence of active MMPs in the intact tissue. In vitro conversion of adipogenic 3T3-F442A cells into mature adipocytes was associated with substantial modulations of MMP and TIMP expression. Moreover, this in vitro adipogenesis was reduced in the presence of a synthetic MMP inhibitor. Thus, the adipose tissue expresses a large array of MMPs and TIMPs, which modulate adipocyte differentiation. |
| Databáze: | OpenAIRE |
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