Membrane disruptive antimicrobial potential of NK-lysin from yellow catfish (Pelteobagrus fulvidraco)
| Autor: | Jing-Jing Song, Xiao-Xiao Liu, Yeqing Wu, Yin-Sheng Wu, Rong Zhu, Xue Lv, Xingguo Wang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Fish Proteins
Lipopolysaccharides 0301 basic medicine Proteolipids Lysin Peptide Aquatic Science Biology Gram-Positive Bacteria Pelteobagrus complex mixtures Microbiology 03 medical and health sciences Gram-Negative Bacteria Animals Environmental Chemistry Edwardsiella ictaluri Pathogen Catfishes chemistry.chemical_classification Effector Cell Membrane 04 agricultural and veterinary sciences General Medicine Antimicrobial biology.organism_classification Poly I-C 030104 developmental biology chemistry 040102 fisheries bacteria 0401 agriculture forestry and fisheries Antimicrobial Cationic Peptides Catfish |
| Zdroj: | Fish & Shellfish Immunology. 97:571-580 |
| ISSN: | 1050-4648 |
| Popis: | NK-lysins, a type of broad-spectrum antimicrobial peptide (AMP), act as an essential effector of innate defense against microbial attack in higher vertebrates and so in fish. The present study delineates the structural and functional characterization of NK-lysin from yellow catfish (Pelteobagrus fulvidrac) (Pelteobagrus fulvidraco). PfNK-lysin encodes a 153-residue peptide, which displays the hallmark features of other known NK-lysins with the ordered array of six well-conserved cysteine residues and five-exon/four-intron structure. It was found to be ubiquitous in tissues, being detected most abundantly in gill and head kidney. In vivo exposure to stimuli (LPS, PolyI:C, and Edwardsiella ictaluri) induced PfNK-lysin expression in head kidney and spleen. Synthetic PfNK-lysin-derived peptide exhibited in vitro bactericidal potency against both Gram-positive and Gram-negative bacteria, with the highest inhibitory effect on pathogen Edwardsiella ictaluri. Fluorescence microscopy and scanning electron microscopy further confirmed its capacity to cause damage to the bacterial plasma membrane. Taken together, these data suggest that PfNK-lysin might participate in antimicrobial defense of yellow catfish by membrane-disruptive action. |
| Databáze: | OpenAIRE |
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