Systematic screening of BK virus by real-time PCR prevents BK virus associated nephropathy in renal transplant recipients
| Autor: | John Wahlgren, Kerstin I. Falk, Björn Öqvist, Anna-Lena Hammarin |
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| Rok vydání: | 2011 |
| Předmět: |
Adult
Male viruses Viremia Biology Real-Time Polymerase Chain Reaction medicine.disease_cause Nephropathy Virology medicine Humans Mass Screening Aged Retrospective Studies Polyomavirus Infections Transplantation Clinical Laboratory Techniques Surrogate endpoint Retrospective cohort study Middle Aged Viral Load medicine.disease Kidney Transplantation BK virus Tumor Virus Infections Infectious Diseases Real-time polymerase chain reaction Renal transplant BK Virus Female Kidney Diseases Viral load |
| Zdroj: | Journal of Medical Virology. 83:1959-1965 |
| ISSN: | 0146-6615 |
| Popis: | BK virus associated nephropathy occurs in approximately 5% of renal transplant recipients. Quantitation of BKV DNA in serum/plasma early in the course of disease has been suggested to be an important diagnostic tool for polymavirus-associated nephropathy (PVAN). The aim of this study was to develop a BKV real-time PCR (qPCR), which could be included in a diagnostic qPCR platform. Additionally, the significance of the assay as a surrogate marker for PVAN was investigated. Quantitation of BKV DNA by qPCR was carried out on 234 serum samples from a retrospective study including 31 renal transplant recipients monitored for at least 6 months post-transplantation. BKV viremia was detected in 9 out of 31 patients. Four patients had a viral load of >10,000 copies/ml at least on one occasion. In two of these patients, PVAN was diagnosed clinically during the study period. In retrospect, these patients were shown to be BKV positive before the clinical diagnosis of PVAN was made. Another two patients had a permanent graft dysfunction, but were never clinically diagnosed with PVAN. None of the remaining five patients with BKV DNA ( |
| Databáze: | OpenAIRE |
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