A zebrafish reporter line reveals immune and neuronal expression of endogenous retrovirus

Autor: Holly A. Rutherford, Amy Clarke, Emily V. Chambers, Jessica J. Petts, Euan G. Carson, Hannah M. Isles, Alejandra Duque-Jaramillo, Stephen A Renshaw, Jean-Pierre Levraud, Noémie Hamilton
Přispěvatelé: The Bateson Centre [Sheffield, UK], University of Sheffield [Sheffield], Institute of Microbiology [University of Lausanne] (IMUL), Centre Hospitalier Universitaire Vaudois [Lausanne] (CHUV), Macrophages et Développement de l’Immunité, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), The Institute of Neuroscience [Sheffield], This work was supported by a European Leukodystrophy Association fellowship (Association Européenne contre les Leucodystrophies, ELA 2016-012F4 to N.H.), a Medical Research Council Programme Grant (MR/M004864/1 to S.A.R.), a studentship from the MRC Discovery Medicine North (DiMeN) Doctoral Training Partnership (MR/N013840/1) and a Sir Jules Thorn PhD scholarship to H.A.R., and an Agence Nationale de la Recherche grant (ANR-16-CE20-0002-03 to J.-P.L.). Open Access funding provided by The University of Sheffield Bateson Centre. Deposited in PMC for immediate release., ANR-16-CE20-0002,Fish-RNAvax,Vaccins ARN éco-compatibles pour l'induction de réponses immunitaires protectrices chez le poisson d'élevage(2016)
Rok vydání: 2021
Předmět:
Zdroj: Disease Models & Mechanisms
Disease Models & Mechanisms, 2022, 15 (4), pp.dmm.048921. ⟨10.1242/dmm.048921⟩
ISSN: 1754-8403
1754-8411
Popis: Endogenous retroviruses (ERVs) are fossils left in our genome from retrovirus infections of the past. Their sequences are part of every vertebrate genome and their random integrations are thought to have contributed to evolution. Although ERVs are mainly kept silenced by the host genome, they are found activated in multiple disease states such as auto-inflammatory disorders and neurological diseases. What makes defining their role in health and diseases challenging is the numerous copies in mammalian genomes and the lack of tools to study them. In this study, we identified 8 copies of the zebrafish endogenous retrovirus (zferv). We created and characterised the first in vivo ERV reporter line in any species. Using a combination of live imaging, flow cytometry and single cell RNA sequencing, we mapped zferv expression to early T cells and neurons. Thus, this new tool identified tissues expressing ERV in zebrafish, highlighting a potential role of ERV during brain development and strengthening the hypothesis that ERV play a role in immunity and neurological diseases. This transgenic line is therefore a suitable tool to study the function of ERV in health and diseases. Funding This work has been supported by a European Leukodystrophy Association fellowship (ELA 2016-012F4) to NH, an MRC Programme Grant (MR/M004864/1) to SAR and . JPL is supported by Agence Nationale de la Recherche (grant ANR-16-CE20-0002-03.. Imaging was carried out in the Wolfson Light Microscopy Facility, supported by an MRC grant (G0700091) and a Wellcome Trust grant (GR077544AIA). Conflict of Interest Statement The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Databáze: OpenAIRE
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