Porcine chromaffin cells, culture, and transplant for antinociceptive effects in rodents and primates
| Autor: | Ying Lu, David C. Yeomans, George D. Pappas, Runfeng Jing |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Nicotine Time Factors Cell Transplantation Swine Xenotransplantation medicine.medical_treatment Chromaffin Cells Transplantation Heterologous Pain relief Radioimmunoassay Pain Biology In Vitro Techniques Rats Sprague-Dawley Retrovirus Catecholamines Internal medicine medicine Animals Bovine adrenal Nicotinic Agonists Cells Cultured Chromatography High Pressure Liquid Pain Measurement Behavior Animal General Medicine Enkephalins Haplorhini biology.organism_classification Rats Transplantation medicine.anatomical_structure Nociception Endocrinology Neurology Chromaffin cell Neurology (clinical) Adrenal chromaffin |
| Zdroj: | Neurological research. 26(7) |
| ISSN: | 0161-6412 |
| Popis: | It has been shown that xenografts and allografts of spinally transplanted adrenal chromaffin cells produce antinociception in animals and pain relief in patients with cancer pain. As there is a very limited availability of human adrenal tissue to serve as allografts, the clinical need for xenogeneic chromaffin cells as transplants is obvious. Bovine adrenal glands as a steady source of chromaffin cells have been extensively studied. There is however concern about the possible infection in humans with retrovirus following transplantation. The purpose of this study is to use the pig as a preferred donor animal species for xenotransplantation into rat and monkey. As pigs have been cloned, this opens the door to gene-targeted technologies and allows for genetic modifications, which possibly could improve the efficacy and safety of chromaffin cell transplantation. Porcine chromaffin cells were isolated from adrenal glands of 6-8-month-old pigs. After culturing cells for 1 week in a medium containing serum, the release of met-enkephalin and norepinephrine from the cells was detected by high-performance liquid chromatography and radioimmunoassay with nicotine stimulation, lasting approximately 3 weeks. Transplantation of these cells into the subarachnoid space of rats produced antinociceptive effects on Adelta and C fiber-mediated responses lasting 2-3 weeks. Similar findings were observed in studies with macaque monkeys. Compared with the same number of bovine chromaffin cells, porcine chromaffin cells showed a more robust and longer antinociceptive effect, and could be a better source of cells for human transplantation. |
| Databáze: | OpenAIRE |
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