Isolation and Abiotic Stress Resistance Analyses of a Catalase Gene fromIpomoea batatas(L.) Lam
| Autor: | Bin Yong, Huanhuan Shao, Qinqin Ma, Yuzhi Miao, Xiaoyan Wang, Zixi Hong, Yusong Jiang, Haiyan Zheng, Xueting Fei, Pan Xu, Xiang-Hua Yuan |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Article Subject Drought tolerance lcsh:Medicine medicine.disease_cause Ipomoea Gene Expression Regulation Enzymologic General Biochemistry Genetics and Molecular Biology Open Reading Frames 03 medical and health sciences Gene Expression Regulation Plant Stress Physiological medicine Ipomoea batatas Escherichia coli Plant Proteins Abiotic component General Immunology and Microbiology biology Abiotic stress lcsh:R food and beverages Sequence Analysis DNA General Medicine Peroxisome Catalase biology.organism_classification Recombinant Proteins Plant Leaves Plant Tubers Open reading frame 030104 developmental biology Biochemistry biology.protein Research Article |
| Zdroj: | BioMed Research International, Vol 2017 (2017) BioMed Research International |
| ISSN: | 2314-6141 2314-6133 |
| Popis: | As an indicator of the antioxidant capability of plants, catalase can detoxify reactive oxygen species (ROS) generated by environmental stresses. Sweet potato is one of the top six most important crops in the world. However, its catalases remain largely unknown. In this study, a catalase encoding gene,IbCAT2(accession number: KY615708), was identified and cloned from sweet potato cv. Xushu 18. It contained a 1479 nucleotides’ open reading frame (ORF). S-R-L, Q-K-L, and a putative calmodulin binding domain were located at the C-terminus of IbCAT2, which suggests that IbCAT2 could be a peroxisomal catalase. Next-generation sequencing (NGS) based quantitative analyses showed thatIbCAT2was mainly expressed in young leaves and expanding tuberous roots under normal conditions. When exposed to 10% PEG6000 or 200 mmol/L NaCl solutions,IbCAT2was upregulated rapidly in the first 11 days and then downregulated, although different tissues showed different degree of change. Overexpression ofIbCAT2conferred salt and drought tolerance inEscherichia coliandSaccharomyces cerevisiae. The positive response ofIbCAT2to abiotic stresses suggested thatIbCAT2might play an important role in stress responses. |
| Databáze: | OpenAIRE |
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