Effective Preparation Method of Phosphopeptides from Phosvitin and the Analysis of Peptide Profiles Using Tandem Mass Spectrometry
| Autor: | Xi Huang, Sun Hee Moon, Hyun-Dong Paik, Eun Joo Lee, Jae-Hoon Lee, Dong U. Ahn, Byungrok Min |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Phosphopeptides
0106 biological sciences Peptide Phosvitin Tandem mass spectrometry 01 natural sciences Hydrolysis Tandem Mass Spectrometry Enzymatic hydrolysis medicine Animals Trypsin Amino Acid Sequence chemistry.chemical_classification Chromatography Chemistry 010401 analytical chemistry Analytic Sample Preparation Methods General Chemistry 0104 chemical sciences Amino acid Enzyme Biocatalysis Peptides General Agricultural and Biological Sciences Chickens 010606 plant biology & botany medicine.drug |
| Zdroj: | Journal of Agricultural and Food Chemistry. 67:14086-14101 |
| ISSN: | 1520-5118 0021-8561 |
| Popis: | The effect of high-temperature and mild-pressure (HTMP) pretreatment on the enzymatic hydrolysis of phosvitin and the structural characteristics of the phosphopeptides produced were analyzed using tandem mass spectrometry. The HTMP pretreatment hydrolyzed phosvitin at random sites and helped the subsequent enzyme hydrolysis of the peptides produced. With the HTMP pretreatment alone, 154 peptides were produced, while the use of trypsin, Protex 6L, and Multifect 14L in combination with the pretreatment produced 252, 280, and 164 peptides, respectively. The use of two enzyme combinations (trypsin + Protex 6L and trypsin + Multifect 14L) helped the hydrolysis further. The number of phosphopeptides produced increased when the modifications within the same amino acid sequences were considered. This study indicated that HTMP pretreatment was a breakthrough method to improve the enzymatic hydrolysis of phosvitin that enabled an easy production of phosvitin phosphopeptides for their subsequent functional characterizations. |
| Databáze: | OpenAIRE |
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