Simultaneous determination of levodopa, carbidopa, entacapone, tolcapone, 3-O-methyldopa and dopamine in human plasma by an HPLC-MS/MS method
| Autor: | Rômulo Pereira, Ribeiro, João Cleverson, Gasparetto, Raquel, de Oliveira Vilhena, Thais Martins, Guimarães de Francisco, Thais Martins Guimarães, de Francisco, Cleverson Antônio Ferreira, Martins, Marco André, Cardoso, Roberto, Pontarolo, Katherine Athayde Teixeira, de Carvalho |
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| Rok vydání: | 2015 |
| Předmět: |
Quality Control
Levodopa Formic acid Dopamine Clinical Biochemistry Catechols High-performance liquid chromatography Analytical Chemistry Nitrophenols chemistry.chemical_compound Benzophenones Tandem Mass Spectrometry Nitriles medicine Humans Entacapone General Pharmacology Toxicology and Pharmaceutics Chromatography High Pressure Liquid Chromatography Tolcapone Carbidopa Reproducibility of Results General Medicine Dihydroxyphenylalanine Medical Laboratory Technology chemistry Tyrosine 3-O-Methyldopa Blood Chemical Analysis medicine.drug |
| Zdroj: | Bioanalysis. 7(2) |
| ISSN: | 1757-6199 |
| Popis: | Background: In this study, we developed and validated a HPLC–MS/MS method capable of simultaneously determining levodopa, carbidopa, entacapone, tolcapone, 3-O-methyldopa and dopamine in human plasma. Results & methodology: Chromatographic separation was achieved using a C8 column with a mobile phase consisting of a gradient of water and acetonitrile:methanol (90:10 v/v), both containing 0.1% formic acid. The developed method was selective, sensitive (LD -1), linear (r > 0.99), precise (RSD ®. Conclusion: The new method can be used for the clinical monitoring of these substances and applied to adjustments in drug dosages. |
| Databáze: | OpenAIRE |
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