Sensitive Detection ofPlasmodium vivaxin Blood by a Cell-Elisa Using a Monoclonal Antibody
| Autor: | Carmen Bracho, Hilda A. Pérez |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Pharmacology
biology medicine.drug_class Immunology Plasmodium vivax Antibodies Monoclonal Enzyme-Linked Immunosorbent Assay Plasmodium falciparum biology.organism_classification medicine.disease Monoclonal antibody Virology Molecular biology Giemsa stain Blood parasitic diseases biology.protein medicine Animals Protozoa Antibody Malaria Peroxidase |
| Zdroj: | Journal of Immunoassay. 17:155-174 |
| ISSN: | 0197-1522 |
| DOI: | 10.1080/01971529608005786 |
| Popis: | An IgM monoclonal antibody (7C5B71) which reacted with the blood stages of Plasmodium vivax, but not with those of Plasmodium falciparum was used in a cell-ELISA to detect parasites in samples of peripheral blood. Blood thin smears were probed with monoclonal antibody 7C5B71 and then reacted with a peroxidase conjugate of the appropriate specificity and the insoluble chromogen amino-ethyl-carbazole. Infected cells which appeared dark red coloured were rapidly identified under a light microscope using a low magnification. The conventional microscopic examination of thin films coloured with Giemsa was used as reference test. Under laboratory conditions the test showed a positive result in samples with a level of parasitaemia ofor = 500 parasites/microliter of blood. In a preliminary field trial the test showed 100 % specificity for the diagnosis of P. vivax malaria. |
| Databáze: | OpenAIRE |
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