Serum miR‐515‐3p, a potential new RNA biomarker, is involved in gastric carcinoma
| Autor: | Hengyu Zhao, Xing Li, Xue Han, Zhongqi Cui, Jianan Zhang, Xiaoyu Ma, Danyang Zhou, Banghao Sun, Lijie Yuan, Anqi Liu |
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| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Oncology medicine.medical_specialty Biology Sensitivity and Specificity Biochemistry law.invention 03 medical and health sciences 0302 clinical medicine Stomach Neoplasms law Internal medicine microRNA Biomarkers Tumor medicine Humans Gene Regulatory Networks KEGG Molecular Biology Gene Early Detection of Cancer Polymerase chain reaction Receiver operating characteristic Gene Expression Profiling Area under the curve Cancer Cell Biology medicine.disease Survival Analysis Up-Regulation Biomarker (cell) Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology ROC Curve Case-Control Studies 030220 oncology & carcinogenesis Female |
| Zdroj: | Journal of Cellular Biochemistry. 120:15834-15843 |
| ISSN: | 1097-4644 0730-2312 |
| Popis: | OBJECTIVES microRNAs (miRNAs) have provided a new opportunity for developing diagnostic biomarkers and effective therapeutic targets in gastric cancer (GC). In this study, we aimed to investigate the relationship between miR-515-3p and GC development. EXPERIMENTAL DESIGN The Gene Expression Omnibus (GEO) database was used for screening genes and miRNA and for 2R analysis. miRNA prediction target genes and screening key genes were analyzed using protein interactions (PPI) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. A network of miRNA-mRNA interactions was predicated by Cytoscape (v.3.5.1), Institute of Systems Biology & University of California, San Diego & Pasteur institute & University of California, San Francisco. Finally, miR-515-3p levels were detected by quantitative reverse-transcription polymerase chain reaction (qRT-PCR) in gastric cells and plasma levels. Then, the association between the expression level of miR-515-3p and the clinicopathological features of patients with GC was further analyzed. OBSERVATIONS AND CONCLUSIONS We found that miR-515-3p was markedly overexpressed in individuals with GC compared with that in normal gastric cells (NCs) and the surgery group (P |
| Databáze: | OpenAIRE |
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