Anti-inflammatory and anti-oxidative effect of curcumin in connective tissue type mast cell
Autor: | Jinsyo Tsutsumi, Hirofumi Nishikawa, Seiichi Kitani |
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Jazyk: | angličtina |
Rok vydání: | 2013 |
Předmět: |
Inflammation
Nutrition and Dietetics Curcumin Chemistry IgG Nutrition. Foods and food supply Lipid peroxidation Degranulation Medicine (miscellaneous) Connective tissue ROS Pharmacology Substance P Mast cell Cell membrane chemistry.chemical_compound medicine.anatomical_structure Biochemistry medicine Arachidonic acid TX341-641 Cytotoxicity Food Science |
Zdroj: | Journal of Functional Foods, Vol 5, Iss 2, Pp 763-772 (2013) |
ISSN: | 1756-4646 |
Popis: | Curcumin in turmeric from rhizome of plant Curcuma longa was investigated on its anti-inflammatory effect with canine cutaneous mastocytoma mast cells. Though the degranulation induced by neuropeptide substance P, specific mast cell secregtagogue Compound 48/80 and immunoglobulin IgG were reached above 10%, curcumin reduced the release below 4.6% at 10 μM without cytotoxicity. Inhibitory effect of curcumin on [Ca2+]i elevation was also confirmed. ROS induced by Compound 48/80 were evaluated with dichlorodihydrofluorescein diacetate (DCFH-DA), and were lowered by curcumin in dose dependent manner. Cell membrane lipid peroxidation was monitored with diphenyl-1-pyrenylphosphine (DPPP). Curcumin decreased cell membrane lipid peroxidation induced by arachidonic acid in the concentration above 0.01 μM. Curcumin also inhibited the protein tyrosine- and threonine-phosphorylations around 33 and 45 kDa, respectively. Taking these findings into consideration with the inhibitory effects on connective tissue type mast cell, there is a potential use to ameliorate inflammation-related chronic diseases. |
Databáze: | OpenAIRE |
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