Mycobacterium leprae isolates from different sources have identical sequences of the spacer region between the 16S and 23S ribosomal RNA genes
| Autor: | Paul R. Klatser, M. Y. L. De Wit |
|---|---|
| Přispěvatelé: | Other departments |
| Rok vydání: | 1994 |
| Předmět: |
DNA
Bacterial Armadillos Restriction Mapping Mice Nude Microbiology DNA Ribosomal Polymerase Chain Reaction DNA sequencing Cercocebus atys Mice 23S ribosomal RNA Leprosy RNA Ribosomal 16S Sequence Homology Nucleic Acid Genotype Animals Humans Mycobacterium leprae Ribosomal DNA Polymorphism Single-Stranded Conformational Genetics Polymorphism Genetic biology Base Sequence Monkey Diseases Single-strand conformation polymorphism Spacer DNA biology.organism_classification 16S ribosomal RNA Molecular biology RNA Bacterial RNA Ribosomal 23S Genes Bacterial |
| Zdroj: | Microbiology (Reading, England), 140(8), 1983-1987. Society for General Microbiology |
| ISSN: | 1350-0872 |
| Popis: | To test for genotypic variations between different isolates of Mycobacterium leprae, the causative agent of leprosy, the 282 bp spacer region between the 16S and 23S rRNA genes was amplified using PCR, and submitted to single-strand conformation polymorphism (SSCP) analysis. The procedure was optimized using four modified spacer fragments, containing mutations at one, three, four and six positions, respectively. Seventy-five M. leprae isolates from different sources, including isolates from leprosy patients, healthy individuals, armadillos and mouse footpads were identical in the SSCP analysis. DNA sequencing and restriction enzyme analysis performed on four and 40 samples, respectively, confirmed the results obtained with SSCP analysis. |
| Databáze: | OpenAIRE |
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