Verification of osteoblast differentiation on airborne-particle abrasion, large-grit, acid-etched surface of titanium implants regulated by yes-associated protein and transcriptional coactivator with PDZ-binding motif
| Autor: | Xiayan Luo, Hui-Qiang Sun, Mengyang Jiang, Ting Wei, Jiayi Li, Yun Yang, Ting-Song Liu |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Bone sialoprotein
Integrin 03 medical and health sciences 0302 clinical medicine stomatognathic system Osteogenesis Transcription (biology) medicine Gene silencing General Dentistry Titanium Messenger RNA Osteoblasts biology Chemistry fungi Cell Differentiation Osteoblast 030206 dentistry Cell biology RUNX2 medicine.anatomical_structure Cytoplasm biology.protein 030217 neurology & neurosurgery Transcription Factors |
| Zdroj: | Journal of Oral Science. 61:431-440 |
| ISSN: | 1880-4926 1343-4934 |
| DOI: | 10.2334/josnusd.18-0112 |
| Popis: | Although airborne-particle abrasion, large-grit, acid-etched (SLA) surface technology can promote implant osseointegration; its mechanism remains unclear. By preparing the SLA titanium (Ti) plate (SLA Ti) and Polished Ti plate (Polished Ti), this experiment investigates the expression and distribution of the Yes-associated protein (YAP) and transcriptional coactivator with the PDZ-binding motif (TAZ) in MC3T3-E1 cells. In addition, gene YAP and TAZ silencing on the SLA Ti was conducted to observe changes in the osteoblast differentiation markers, runt-related transcription factor-2 (Runx2) and bone sialoprotein (BSP). The results demonstrated that SLA Ti surface microtopography could induce YAP/TAZ's transfer from the cytoplasm to the nuclei of MC3T3-E1 cells. The expression of YAP/TAZ increased in terms of mRNA and protein. After silencing the YAP/TAZ genes, Runx2 and BSP decreased, suggesting that YAP/TAZ plays an important regulatory role in this process. Meanwhile, the results also showed that SLA microtopography enhanced the expression of integrins α1, α2, and β1. After silencing the integrin α1, α2, and β1 genes, YAP and TAZ decreased in terms of mRNA and protein. Therefore, this experiment was the first to confirm that SLA surface microtopography facilitates osteoblast differentiation by regulating YAP/TAZ and confirms that the process can be related to integrins α1, α2, and β1. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|