Bioanalysis of the Enantiomers of (±)-Sarin using Automated Thermal Cold-Trap Injection Combined with Two-Dimensional Gas Chromatography
| Autor: | Helma E.T. Spruit, Hendrik P. Benschop, Jan P. Langenberg, Hendrik C. Trap |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
chemistry.chemical_classification
Detection limit Sarin Chromatography Gas Chemical Health and Safety Chromatography Health Toxicology and Mutagenesis Analytical chemistry Stereoisomerism Toxicology Analytical Chemistry chemistry.chemical_compound chemistry Two-dimensional chromatography Soman Organophosphorus compound medicine Humans Environmental Chemistry Chemical Warfare Agents Solid phase extraction Enantiomer Nerve agent medicine.drug |
| Zdroj: | Journal of Analytical Toxicology. 25:57-61 |
| ISSN: | 1945-2403 0146-4760 |
| DOI: | 10.1093/jat/25.1.57 |
| Popis: | A fully automated multidimensional gas chromatographic system with thermal desorption injection and alkali flame detection was developed for analysis of the enantiomers of the nerve agent ( The chiral stationary phase was CP Cyclodex B on which the sarin enantiomers were completely resolved. The absolute detection limit was 2.5 pg per enantiomer. The method is intended to be used for the analysis of the sarin enantiomers in biological samples. For this purpose, sarin was isolated from guinea pig blood via solid-phase extraction. Deuterated sarin was used as internal standard. Stabilization of sarin in the blood sample by acidification and addition of an excess of a competitive organophosphorus compound (neopentyl sarin) appeared to be essential. The absolute recovery of the extraction procedure was 60%, whereas the recovery relative to the internal standard was 100%. As in the case of C(177 it was considered to be essential to differentiate between the enantiomers of ( Previous investigations in our laboratory have shown that the bimolecular rate constant for inhibition of electric eel AChE by (-)-satin is approximately four orders of magnitude higher than that of (+)-satin (9). Consequently, we expect that (+)sarin, by analogy with the C( of soman, will be |
| Databáze: | OpenAIRE |
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