Reduction of animal suffering in rabies vaccine potency testing by introduction of humane endpoints
| Autor: | Satsuki Kakiuchi, Ichiro Kurane, Chang-Kweng Lim, Madoka Horiya, Guillermo Posadas-Herrera, Kazuo Nakamichi, Mutsuyo Takayama-Ito, Masayuki Saijo |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Time Factors Rabies Physiology Bioengineering Chick Embryo medicine.disease_cause Applied Microbiology and Biotechnology 03 medical and health sciences Mice Rabies vaccine Weight loss Weight Loss medicine Paralysis Potency Animals Humans Adverse effect Vaccine Potency Pharmacology General Immunology and Microbiology business.industry Rabies virus Body Weight Vaccination General Medicine medicine.disease Survival Analysis Survival Rate 030104 developmental biology Rabies Vaccines Vaccines Inactivated Immunology Female medicine.symptom business Biotechnology medicine.drug |
| Zdroj: | Biologicals : journal of the International Association of Biological Standardization. 46 |
| ISSN: | 1095-8320 |
| Popis: | Potency controls of inactivated rabies vaccines for human use are confirmed by the National Institutes of Health challenge test in which lethal infection with severe neurological symptoms should be observed in approximately half of the mice inoculated with the rabies virus. Weight loss, decreased body temperature, and the presence of rabies-associated neurological signs have been proposed as humane endpoints. The potential for reduction of animal suffering by introducing humane endpoints in the potency test for inactivated rabies vaccine for human use was investigated. The clinical signs were scored and body weight was monitored. The average times to death following inoculation were 10.49 and 10.99 days post-inoculation (dpi) by the potency and challenge control tests, respectively, whereas the average times to showing Score-2 signs (paralysis, trembling, and coma) were 6.26 and 6.55 dpi, respectively. Body weight loss of more than 15% appeared at 5.82 and 6.42 dpi. The data provided here support the introduction of obvious neuronal signs combined with a body weight loss of ≥15% as a humane endpoint to reduce the time of animal suffering by approximately 4 days. |
| Databáze: | OpenAIRE |
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