Extracellular Vesicles as Predictors of Individual Response to Exercise Training in Youth Living with Obesity

Autor: Ayesha Saleem, Martin Sénéchal, Jonathan M. McGavock, Keith R. Brunt, Ashley L. Eadie, Hagar I. Labouta, Benjamin Bydak, Samira Seif, Patience O. Obi, Alexandria Martin, Taiana M. Pierdoná
Rok vydání: 2022
Předmět:
Zdroj: Frontiers in bioscience (Landmark edition). 27(5)
ISSN: 2768-6698
Popis: Exercise is associated with health benefits, including the prevention and management of obesity. However, heterogeneity in the adaptive response to exercise training exists. Our objective was to evaluate if changes in extracellular vesicles (EVs) after acute aerobic exercise were associated with the responder phenotype following 6-weeks of resistance training (RT).This is a secondary analysis of plasma samples from the EXIT trial (clinical trial#02204670). Eleven sedentary youth with obesity (15.7 ± 0.5 yrs, BMI ≥95th percentile) underwent acute exercise (60% HRR, 45 min). Blood was collected at baseline [AT0 min], during [AT15-45 min], and 75 min post-recovery [AT120], and EVs purified using size exclusion chromatography from extracted plasma. Afterward, youth participated in 6-weeks RT and were categorized into responders or non-responders based on changes in insulin sensitivity.We assessed EV biophysical profile (size, zeta potential, protein yield, and EV subtype protein expression) in a single-blind fashion. Overall, there was a general increase in EV production in both groups. Average EV size was larger in responders (~147 nm)Our data suggest that youth with obesity that respond to RT produce larger EVs that are TSG101+ and CD63+, with increased EV protein yield during acute exercise.
Databáze: OpenAIRE