Rapid estimation of whole blood everolimus concentrations using architect sirolimus immunoassay and mathematical equations: comparison with everolimus values determined by liquid chromatography/mass spectrometry
| Autor: | Andre Smith, Vanessa Moreno, Shawn Balark, Amitava Dasgupta, Marilyn Sonilal, Charles T. Van Buren, Neelam Tejpal |
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| Rok vydání: | 2011 |
| Předmět: |
Microbiology (medical)
Clinical Biochemistry Models Biological Mass Spectrometry Food and drug administration Mathematical equations Liquid chromatography–mass spectrometry medicine Humans Immunology and Allergy Everolimus cardiovascular diseases Whole blood Immunoassay Sirolimus Chromatography medicine.diagnostic_test Chemistry Biochemistry (medical) Public Health Environmental and Occupational Health Original Articles Hematology equipment and supplies Medical Laboratory Technology Therapeutic drug monitoring Calibration Linear Models Chromatography Liquid medicine.drug |
| Zdroj: | Journal of Clinical Laboratory Analysis. 25:207-211 |
| ISSN: | 0887-8013 |
| DOI: | 10.1002/jcla.20459 |
| Popis: | United States Food and Drug Administration (FDA) in 2010 approved the use of immunosuppressant drug everolimus, which requires therapeutic drug monitoring in whole blood. Taking advantage of structural similarity between sirolimus and everolimus we attempted to rapidly estimate everolimus concentration from apparent sirolimus concentration obtained by using Architect sirolimus immunoassay and mathematical equations (both polynomial and linear). Mathematical equations were derived by curve‐fitting methods based on observed apparent sirolimus concentration and true everolimus concentration determined by a liquid chromatography combined with mass spectrometry (LC/MS) method using eight everolimus standards (concentration range 1–30 ng/mL) prepared in whole blood. In order to determine the validity of our approach, we analyzed 12 specimens from patients receiving everolimus using both Architect sirolimus assay and LC/MS method. We observed good correlation between calculated everolimus values and true everolimus values as determined by LC/MS. However, if a patient is switched from sirolimus to everolimus, then sirolimus immunoassay can roughly estimate everolimus concentration plus any residual sirolimus present in whole blood and it is not possible to calculate everolimus concentration. J. Clin. Lab. Anal. 25:207–211, 2011. © 2011 Wiley‐Liss, Inc. |
| Databáze: | OpenAIRE |
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