Evaluation of Adverse Effects of Resorbable Hyaluronic Acid Fillers: Determination of Macrophage Responses
| Autor: | Wim H. De Jong, Danyel Jennen, Peter H. J. Keizers, Hennie M. Hodemaekers, Jolanda P. Vermeulen, Frank Bakker, Paul Schwillens, Marcel van Herwijnen, Marlon Jetten, Jos C. S. Kleinjans, Robert E. Geertsma, Rob J. Vandebriel |
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| Přispěvatelé: | Toxicogenomics, RS: GROW - R1 - Prevention, CRISP |
| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: |
COMPLICATIONS
Macrophages Organic Chemistry Biocompatible Materials General Medicine Catalysis Computer Science Applications resorbable fillers hyaluronic acid adverse effects macrophage responses Inorganic Chemistry SOFT-TISSUE AUGMENTATION Dermal Fillers Cytokines Humans Physical and Theoretical Chemistry INJECTION Molecular Biology Spectroscopy |
| Zdroj: | International journal of molecular sciences, 23(13):7275. Multidisciplinary Digital Publishing Institute (MDPI) International Journal of Molecular Sciences; Volume 23; Issue 13; Pages: 7275 |
| ISSN: | 1661-6596 |
| Popis: | Resorbable tissue fillers for aesthetic purposes can induce severe complications including product migration, late swelling, and inflammatory reactions. The relation between product characteristics and adverse effects is not well understood. We hypothesized that the degree of cross-linking hyaluronic acid (HA) fillers was associated with the occurrence of adverse effects. Five experimental HA preparations similar to HA fillers were synthesized with an increasing degree of cross-linking. Furthermore, a series of commercial fillers (Perfectha®) was obtained that differ in degradation time based on the size of their particulate HA components. Cytotoxic responses and cytokine production by human THP-1-derived macrophages exposed to extracts of the evaluated resorbable HA fillers were absent to minimal. Gene expression analysis of the HA-exposed macrophages revealed the responses related to cell cycle control and immune reactivity. Our results could not confirm the hypothesis that the level of cross-linking in our experimental HA fillers or the particulate size of commercial HA fillers is related to the induced biological responses. However, the evaluation of cytokine induction and gene expression in macrophages after biomaterial exposure presents promising opportunities for the development of methods to identify cellular processes that may be predictive for biomaterial-induced responses in patients. |
| Databáze: | OpenAIRE |
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