Use of an artificial oxygen carrier in isolated rat liver perfusion: first demonstration of net glucose uptake at physiological portal glucose concentrations using a hemoglobin-free perfusate
| Autor: | M. Plauth, F. Hartmann, Bauder-Gross D, Zimmermann B, D. Vieillard-Baron, Armin Raible |
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| Rok vydání: | 1991 |
| Předmět: |
Male
medicine.medical_specialty Glucose uptake chemistry.chemical_element Carbohydrate metabolism Oxygen Blood Substitutes In vivo Internal medicine medicine Animals Hypoxia Fluorocarbons Chemistry Substrate (chemistry) Rats Inbred Strains General Medicine Metabolism Aerobiosis Rats Perfusion Portal System Glucose Endocrinology Liver Hemoglobin Glycogen |
| Zdroj: | Research in Experimental Medicine. 191:339-347 |
| ISSN: | 1433-8580 0300-9130 |
| DOI: | 10.1007/bf02576689 |
| Popis: | A defect in isolated perfused rat-liver (IPRL) preparations has been proposed to explain discrepancies between in vivo and in vitro findings regarding hepatic glucose metabolism. The aim of the present study was to investigate whether a preparation of IPRL using a synthetic hemoglobin-free perfusate was capable of net glucose uptake and glycogen deposition at physiological portal substrate concentrations. Livers from fed anaesthetized rats were perfused in a recirculating system using a fluorocarbon emulsion as artificial oxygen carrier. Depending on the prevailing glucose concentration, livers exhibited net glucose uptake or release with a threshold value of 5.5-6.0 mM glucose. Net glucose uptake was associated with net glycogen deposition (+0.23 to +0.59 mumol C6 min-1 g-1). From 5.8 mM (n = 3) and 10.0 mM (n = 8), initial concentration glucose levels fell to 5.3 +/- 0.2 mM after 210 min (n = 3) and 6.3 +/- 0.9 mM after 120 min (n = 8), respectively. This was equivalent to a net glucose uptake of -0.16 and -0.45 mumol min-1 g-1. Anoxia reversibly switched hepatic glucose balance from net uptake (-0.42 mumol min-1 g-1) to release (+0.69 mumol min-1 g-1) followed by net uptake (-0.50 mumol min-1 g-1) after reinstitution of aerobic conditions. We conclude that the composition of perfusion media might play a pivotal role for studies of glucose metabolism in the isolated perfused rat liver. In our experimental model, using a hemoglobin-free synthetic medium, net glucose uptake was readily demonstrated at physiological portal substrate concentrations similar to the in vivo situation. |
| Databáze: | OpenAIRE |
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