Dose dependent effects of chronic exposure to toluene on neuronal and glial cell marker proteins in the central nervous system of rats
Autor: | Yasuhiro Takeuchi, Eiji Shibata, K. Kato, Y. Ono, Naomi Hisanaga, Nobuyuki Asaeda, Jiang Huang |
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Rok vydání: | 1992 |
Předmět: |
Male
medicine.medical_specialty Cerebellum Calbindins Central nervous system Biology Creatine Stem cell marker chemistry.chemical_compound S100 Calcium Binding Protein G Internal medicine medicine Animals Neurons Dose-Response Relationship Drug Public Health Environmental and Occupational Health Brain Proteins Rats Inbred Strains Organ Size Rats Endocrinology medicine.anatomical_structure Biochemistry Gliosis chemistry Calbindin 1 Phosphopyruvate Hydratase Toxicity biology.protein Neuroglia Creatine kinase medicine.symptom Toluene Research Article |
Zdroj: | British journal of industrial medicine. 49(4) |
ISSN: | 0007-1072 |
Popis: | The dose dependent effects of chronic exposure to toluene on the neuronal marker proteins (gamma-enolase, calbindin-D28k) and glial cell marker proteins (alpha-enolase, creatine kinase-B, and beta-S100 protein) were investigated in the central nervous system (CNS) of rats. Three groups of animals were exposed to 100 ppm, 300 ppm, or 1000 ppm toluene vapour eight hours a day, six days a week for 16 weeks. The contents of the marker substances were determined with enzyme immunoassays. A significant increase in the three glial cell marker proteins was noted in the cerebellum after exposure to 100 ppm toluene; a more pronounced increase occurred at the higher toluene concentrations. beta-S100 protein also exhibited a dose dependent increase in the brainstem and spinal cord. On the other hand, the two neuronal cell markers did not show a quantitative decrease in the CNS. This means that the development of gliosis, rather than neurone death, is induced by chronic exposure to toluene. The significant biochemical changes induced around the threshold limit value and the concentration dependent alterations suggest that these nerve specific marker proteins may be used to evaluate solvent related damage to the CNS. |
Databáze: | OpenAIRE |
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