In Silico-Guided Rational Drug Design and Semi-synthesis of C(2)-Functionalized Huperzine A Derivatives as Acetylcholinesterase Inhibitors
| Autor: | Nopporn Thasana, Shisanupong Anukanon, Jaruwan Chatwichien, Wareepat Tiyabhorn, Somsak Ruchirawat, Worawat Niwetmarin, Pornkanok Pongpamorn, Richard B. Sessions |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | ACS Omega Anukanon, S, Pongpamorn, P, Tiyabhorn, W, Chatwichien, J, Niwetmarin, W, Sessions, R B, Ruchirawat, S & Thasana, N 2021, ' In Silico-Guided Rational Drug Design and Semi-synthesis of C(2)-Functionalized Huperzine A Derivatives as Acetylcholinesterase Inhibitors ', ACS Omega, vol. 6, no. 30, pp. 19924-19939 . https://doi.org/10.1021/acsomega.1c02875 ACS Omega, Vol 6, Iss 30, Pp 19924-19939 (2021) |
| ISSN: | 2470-1343 |
| Popis: | Huperzine A (1, Hup A), a lycodine-type Lycopodium alkaloid isolated from Thai clubmosses Huperzia squarrosa (G. Forst.) Trevis., H. carinata (Desv. ex. Poir.) Trevis., H. phlegmaria (L.), and Phlegmariurus nummulariifolius (Blume) Chambers (Lycopodiaceae), exerts inhibitory activity on acetylcholinesterase, a known target for Alzheimer's disease therapy. This study investigated the structure-activity relationship of C(2)-functionalized and O- or N-methyl-substituted huperzine A derivatives. In silico-guided screening was performed to search for potential active compounds. Molecular docking analysis suggested that substitution at the C(2) position of Hup A with small functional groups could enhance binding affinity with AChE. Consequently, 12 C(2)-functionalized and four O- or N-methyl-substituted compounds were semi-synthesized and evaluated for their eeAChE and eqBChE inhibitory activities. The result showed that 2-methoxyhuperzine A (10) displayed moderate to high eeAChE inhibitory potency (IC50 = 0.16 μM) with the best selectivity over eqBChE (selectivity index = 3633). Notably, this work showed a case of which computational analysis could be utilized as a tool to rationally screen and design promising drug molecules, getting rid of impotent molecules before going more deeply on labor-intensive and time-consuming drug discovery and development processes. |
| Databáze: | OpenAIRE |
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