Efeito da tibolona sobre o endométrio de ratas castradas
| Autor: | Helene Nara Henriques, Maria Angélica Guzmán-Silva, Ana Carolina Bergmann de Carvalho, José Augusto Soares Pantaleão, Porphirio José Soares Filho, Carlos Eduardo Pollastri |
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| Jazyk: | portugalština |
| Rok vydání: | 2009 |
| Předmět: |
medicine.medical_specialty
Uterus Physiology Tibolone Endometrium Endométrio/metabolismo Ratos Wistar Endométrio/histologia Norpregnenos/dosagem Internal medicine medicine Endométrio/anatomia business.industry Obstetrics and Gynecology Uterine horns Hyperplasia medicine.disease Castrated Female Epithelium Squamous metaplasia medicine.anatomical_structure Endocrinology Norpregnenos/administração business medicine.drug Menopausa/efeitos de drogas |
| Zdroj: | Revista Brasileira de Ginecologia e Obstetrícia v.31 n.3 2009 Revista brasileira de ginecologia e obstetrícia Federação Brasileira das Sociedades de Ginecologia e Obstetrícia (FEBRASGO) instacron:FEBRASGO |
| Popis: | PURPOSE: to evaluate the effect of long-term use of a high dose of tibolone on the morphology of the endometrium of castrated female rats. METHODS: fifteen female Wistar rats, aged eight weeks and weighting about 250 g were used. All the female rats were submitted to bilateral oophorectomy and 30 days afterwards, vaginal cytology was collected, to verify the menopause status. The female rats were randomly divided in two groups. The Treatment Group (n=9) received 1 mg of tibolone/day orally; the Control Group (n=6) received a solution of carboxymethylcellulose vehicle. After 20 weeks of treatment, all the animals were sedated and sacrificed by cervical dislocation. The uterus was removed and fixated in 10% buffer formaldehyde. Both uterine horns were divided in three regions (proximal, medial and distal) and processed to be included in paraffin. Histological sections, stained with hematoxylin-eosin were submitted to morphological and morphometrical analysis. The following parameters have been analyzed: thickness of the endometrial superficial epithelium, thickness of the endometrium stroma, endometrial area, absolute number of endometrial glands and number of glands/endometrial area. The data obtained were compared by the t-Student test. RESULTS: in the Tibolone Group, the uteri were well developed and there was a significant increase (p |
| Databáze: | OpenAIRE |
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