Quantitation of the Cytokines TNF- , IL-8 and IL-10 in Bovine Milk Using Real-Time TaqMan® PCR
| Autor: | A. Luciani, P. De Santis, A. Polci, Angelo Peli, D. Britti |
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| Přispěvatelé: | A. Peli, A. Luciani, P. De Santi, A. Polci, D. Britti |
| Rok vydání: | 2004 |
| Předmět: |
Bovine milk
DAIRY COW Staphylococcus Corynebacterium Biology Polymerase Chain Reaction MASTITIS TaqMan medicine Animals Lactation RNA Messenger Interleukin 8 General Veterinary Tumor Necrosis Factor-alpha Interleukin-8 CYTOKINES Streptococcus General Medicine medicine.disease Molecular biology Interleukin-10 Mastitis Interleukin 10 Milk REAL-TIME TAQMAN® PCR Cattle Female |
| Zdroj: | Veterinary Research Communications. 28:359-362 |
| ISSN: | 0165-7380 |
| DOI: | 10.1023/b:verc.0000045446.26734.ab |
| Popis: | The expression of the TNF-a, IL-8 and IL-10 cytokines in somatic milk cells harvested from 15 dairy cows, was determined by Real-Time TaqMan® reverse transcriptase-polymerase chain reaction (real-time TaqMan® RT-PCR) method. Values of TNF-a were found to be higher in samples from mastitic than from healthy cows, and showed no relationship to the Somatic Cell Count (SCC). On the contrary, the chemotactic factor IL-8 was shown to be higher in samples with high SCC, but showed no relationship to the presence of bacteria in the mammary gland. The results demonstrated that the presence of bacteria appeared to stimulate the synthesis of TNF-a but not that of IL-8 in the bovine udder. |
| Databáze: | OpenAIRE |
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