Quantitation of IgG antibody to Streptococcus pneumoniae vaccine by ELISA and FAST-ELISA using tyraminated antigen
| Autor: | Noori E. Barka, Fouad N. Boctor, Melkon S. Agopian |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Immunology
Dose-Response Relationship Immunologic Tyramine Enzyme-Linked Immunosorbent Assay Immunoglobulin E medicine.disease_cause Incubation period Antigen Streptococcus pneumoniae medicine Immunology and Allergy Humans Antigens Bacterial biology Polysaccharides Bacterial Radioimmunoassay Streptococcaceae biology.organism_classification Virology Antibodies Bacterial Streptococcus pneumoniae vaccine Immunoglobulin G Bacterial Vaccines biology.protein Immunization Antibody |
| Zdroj: | Journal of immunological methods. 120(2) |
| ISSN: | 0022-1759 |
| Popis: | We examined two ELISA methods for measuring antibodies to Streptococcus pneumoniae using tyraminated S. pneumoniae polysaccharide types 3, 7N, 9F and 14 as antigens. The ELISA has the usual format with a relatively long incubation time whereas the FAST-ELISA has a short incubation time and employs a different solid-phase configuration. We showed that both techniques can be used for the detection of antibodies to S. pneumoniae polysaccharides. Although its analytical sensitivity is about 1/10 of that of the ELISA, the FAST-ELISA is sufficiently sensitive to distinguish protective from unprotective levels of antibodies to the types of S. pneumoniae studied. In studying pre- and post-immunization response, we showed that type 3 is the most immunogenic. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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