ChIP-seq analysis of the LuxR-type regulator VjbR reveals novel insights into the Brucella virulence gene expression network
| Autor: | Rodrigo Sieira, Hernán Ruy Bonomi, Claudia L. Kleinman, Romina M. Rodríguez, Gabriela Sycz, Angeles Zorreguieta |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Chromatin Immunoprecipitation Transcription Genetic Gene regulatory network Regulator Repressor Virulence Brucella abortus Biology Ciencias Biológicas purl.org/becyt/ford/1 [https] 03 medical and health sciences Biología Celular Microbiología Bacterial Proteins Genetics Transcriptional regulation Vjbr Gene Regulatory Networks Nucleotide Motifs purl.org/becyt/ford/1.6 [https] Gene Transcription factor Luxr-Type Transcriptional Regulation Binding Sites Brucella Abortus Gene regulation Chromatin and Epigenetics High-Throughput Nucleotide Sequencing Quorum Sensing Gene Expression Regulation Bacterial Bioquímica y Biología Molecular Repressor Proteins 030104 developmental biology Trans-Activators Infection Chromatin immunoprecipitation CIENCIAS NATURALES Y EXACTAS Transcription Factors Protein Binding |
| Zdroj: | Nucleic Acids Research CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| ISSN: | 1362-4962 0305-1048 |
| Popis: | LuxR-type transcription factors control diverse physiological functions necessary for bacterial adaptation to environmental changes. In the intracellular pathogen Brucella, the LuxR homolog VjbR has been shown to regulate the expression of virulence factors acting at early stages of the intracellular infection and, directly or indirectly, hundreds of additional genes. However, the precise determination of VjbR direct targets has so far proved elusive. Here, we performed chromatin immunoprecipitation of VjbR followed by next-generation sequencing (ChIP-seq). We detected a large amount of VjbR-binding sites distributed across the Brucella genome and determined a markedly asymmetric binding consensus motif, an unusual feature among LuxR-type regulators. RNA-seq analysis performed under conditions mimicking the eukaryotic intracellular environment revealed that, among all loci associated to VjbR-binding, this regulator directly modulated the expression of only a subset of genes encoding functions consistent with an intracellular adaptation strategy for survival during the initial stages of the host cell infection. Other VjbR-binding events, however, showed to be dissociated from transcription and may require different environmental signals to produce a transcriptional output. Taken together, our results bring new insights into the extent and functionality of LuxR-type-related transcriptional networks Fil: Kleinman, Claudia Laura. Mc Gill University. Lady Davis Research Intitute; Fil: Sycz, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Fil: Bonomi, Hernán Ruy. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Fil: Rodríguez, Romina M.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Fil: Zorreguieta, Ángeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Fil: Sieira, Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires |
| Databáze: | OpenAIRE |
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