By-Products of the Black Soybean Sauce Manufacturing Process as Potential Antioxidant and Anti-Inflammatory Materials for Use as Functional Foods
| Autor: | Yi-Wen Shih, Chih-Chung Wu, Ying-Ming Chiu, Shu-Ling Hsieh, Chien-Chun Li, Shao-Feng Tseng |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Antioxidant
antioxidant medicine.drug_class DPPH Thiobarbituric acid medicine.medical_treatment Plant Science Anti-inflammatory Article Nitric oxide chemistry.chemical_compound by-product medicine TBARS Food science Ecology Evolution Behavior and Systematics chemistry.chemical_classification Reactive oxygen species black bean soybean sauce inflammation functional supplements ABTS Ecology Botany chemistry QK1-989 lipids (amino acids peptides and proteins) |
| Zdroj: | Plants Plants, Vol 10, Iss 2579, p 2579 (2021) Plants; Volume 10; Issue 12; Pages: 2579 |
| ISSN: | 2223-7747 |
| Popis: | To assess the potential of by-products of the black bean fermented soybean sauce manufacturing process as new functional food materials, we prepared black bean steamed liquid lyophilized product (BBSLP) and analysed its antioxidant effects in vitro. RAW264.7 macrophages were cultured and treated with BBSLP for 24 h, and 1 μg/mL lipopolysaccharide (LPS) was then used for another 24 h to induce inflammation. The cellular antioxidant capacity and inflammatory response were then analysed. Activation of nuclear factor kappa B (NF-κB) signaling in RAW264.7 macrophages was also analysed. Results showed BBSLP had 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium (ABTS+) radical-scavenging abilities and reducing power in vitro. The levels of both reactive oxygen species (ROS) and thiobarbituric acid reactive substances (TBARS) were reduced after RAW264.7 macrophages were treated with BBSLP after LPS induction. After RAW264.7 macrophage treatment with BBSLP and induction by LPS, the levels of inflammatory molecules, including nitric oxide (NO), prostaglandin E2 (PGE2), IL-1α, IL-6 and TNF-α, decreased. NF-κB signaling activity was inhibited by reductions in IκB phosphorylation and NF-κB DNA-binding activity after RAW264.7 macrophages were treated with BBSLP after LPS induction. In conclusion, BBSLP has antioxidant and anti-inflammatory capabilities and can be a supplement material for functional food. |
| Databáze: | OpenAIRE |
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